FREQUENT ALLELIC IMBALANCE AND CYTOGENETIC DELETION ON THE SHORT ARM OF CHROMOSOME 1 IN NASOPHARYNGEAL CARCINOMA

• Published in: Chinese journal of cancer research Vol. 16; no. 1; pp. 5 - 10
• Main Author: 黄铁军 黄必军 张林杰 梁启万 方燕
• Format: Journal Article
• Language: English
• Published: Department of Nuclear Medicine,the Second Municipal Hospital of Shenzhen,Shenzhen 518035%Research Department,Cancer Center,Sun Yat-sen University,Guangzhou 510060 01.03.2004
• Subjects: 染色体; 等位基因; 聚合酶链反应; 遗传病理学; 鼻咽癌; Nasopharyngeal carcinoma; Microsatellite instability; Chromosome lp; Loss of heterozygosity
• ISSN: 1000-9604; 1993-0631
• DOI: 10.1007/BF02974858

Objective: To construct a fine map of the loss of chromosome lpter-p36.11 region in nasopharyngeal carcinoma (NPC) using PCR-LOH technique. Methods:DNA extracted from separated cancerous cells and their mated noncancerous lymphocytes from 47 cases of NPC biopsies were analyzed by means of PCR-LOH to detect 20 loci spanning chromosome lpter-p36.11 region in NPC.Results: In 47 NPC cases, 37 (82.2%) cases showed at least one loci LOH. The highest frequency of less of heterozygosity (LOH) at all 20 loci was found at loci DIS234 (50. 0%) on lp36.13 and loci DIS2644 (37.5%) on lp36.22. There was a statistically significant difference between DIS234 LOH frequency (60%, 9/15) in early stage and that (50.0%, 8/16) in advanced stage (P>0.05). Of all 20 STSs (sequence tqgged-site, STS), DIS243 (37.5%) and DIS199 (30.2%) showed the highest frequency of MI(microsatellite instability) on lp36.33 and lp36.21, respectively. In addition,several cases showed a contiguous stretch of allelic loss in a different level. Conclusion: Two minimal deletion regions (MDR) on the short arm of chromosome 1 were seated at lp36.13(DIS234, 2.0cm) and lp36.22 (DIS436-DIS2644, 6.3cm) respectively, indicating that one or more candidate tumor suppressor gene (TSG) in the two regions may be involved in NPC pathogenesis in an early clinical stage.