Comparative Study of Fluorescence in situ Hybridization Analysis and Karyotype Analysis in Spontaneous Abortion Etiology Diagnosis
Objective To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. Methods A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age. The villi from 100 cases (grou...
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Published in | Journal of reproduction and contraception Vol. 24; no. 3; pp. 133 - 142 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Elsevier B.V
01.09.2013
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Abstract | Objective To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. Methods A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age. The villi from 100 cases (group A) were cultured and used for karyotype analysis, while the uncultured villi from 101 women (group B) were used for FISH. A case was randomly selected from pregnant patients of 6-11 weeks at each gestational week in each group, 6 cases from each group and a total of 12 cases were analyzed by FISH and karyotype at the same time. Results The successful karyotype analysis rate was 66. 0%, and the abnormality karyotype rate was 30.3%; FISH success analysis rate was 100%, and the abnormality rate was 46. 5%; there were significant differences between FISH and karyotype analysis (P=0.036). Conclusion There were obvious differences between the two techniques. FISH was more successfully analyzed, and was used to more precisely determine fetal chromo- some number. It is an effective way to determine abnormal chromosome by integrating FISH and karyotype analysis in spontaneous abortion. |
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AbstractList | To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion.
A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age. The villi from 100 cases (group A) were cultured and used for karyotype analysis, while the uncultured villi from 101 women (group B) were used for FISH. A case was randomly selected from pregnant patients of 6–11 weeks at each gestational week in each group, 6 cases from each group and a total of 12 cases were analyzed by FISH and karyotype at the same time.
The successful karyotype analysis rate was 66.0%, and the abnormality karyotype rate was 30.3%; FISH success analysis rate was 100%, and the abnormality rate was 46.5%; there were significant differences between FISH and karyotype analysis (P=0.036).
There were obvious differences between the two techniques. FISH was more successfully analyzed, and was used to more precisely determine fetal chromosome number. It is an effective way to determine abnormal chromosome by integrating FISH and karyotype analysis in spontaneous abortion. Objective To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. Methods A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age. The villi from 100 cases (group A) were cultured and used for karyotype analysis, while the uncultured villi from 101 women (group B) were used for FISH. A case was randomly selected from pregnant patients of 6-11 weeks at each gestational week in each group, 6 cases from each group and a total of 12 cases were analyzed by FISH and karyotype at the same time. Results The successful karyotype analysis rate was 66. 0%, and the abnormality karyotype rate was 30.3%; FISH success analysis rate was 100%, and the abnormality rate was 46. 5%; there were significant differences between FISH and karyotype analysis (P=0.036). Conclusion There were obvious differences between the two techniques. FISH was more successfully analyzed, and was used to more precisely determine fetal chromo- some number. It is an effective way to determine abnormal chromosome by integrating FISH and karyotype analysis in spontaneous abortion. |
Author | Liang CHANG Su ZHONG Nan ZHAO Ping LIU Jie QIAO |
AuthorAffiliation | Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Key Laboratory of Assisted Reproduction, Ministry of Education, Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China |
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Notes | Objective To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. Methods A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age. The villi from 100 cases (group A) were cultured and used for karyotype analysis, while the uncultured villi from 101 women (group B) were used for FISH. A case was randomly selected from pregnant patients of 6-11 weeks at each gestational week in each group, 6 cases from each group and a total of 12 cases were analyzed by FISH and karyotype at the same time. Results The successful karyotype analysis rate was 66. 0%, and the abnormality karyotype rate was 30.3%; FISH success analysis rate was 100%, and the abnormality rate was 46. 5%; there were significant differences between FISH and karyotype analysis (P=0.036). Conclusion There were obvious differences between the two techniques. FISH was more successfully analyzed, and was used to more precisely determine fetal chromo- some number. It is an effective way to determine abnormal chromosome by integrating FISH and karyotype analysis in spontaneous abortion. 31-1555/R spontaneous abortion; early pregnancy; fluorescence in situ hybridization(FISH); karyotype analysis |
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References | Tian, Wang, Zhang (bib4) 2010; 32 Bell, Van Deerlin, Haddad (bib5) 1999; 71 Xia, Li (bib3) 1989 Xing, Jia, Wu (bib1) 2012; 32 Lei, Wang, Zhou (bib2) 2009; 44 |
References_xml | – volume: 44 start-page: 253 year: 2009 end-page: 256 ident: bib2 article-title: Genetic analysis of chorionic villi specimen in spontaneous abortion using various methods publication-title: Chin J Obstet Gynecol (in Chinese) contributor: fullname: Zhou – volume: 32 start-page: 741 year: 2010 end-page: 742 ident: bib4 article-title: Cytogenetics analysis in sponataneous abortion chorionic villi publication-title: Hebei Medicine contributor: fullname: Zhang – volume: 32 start-page: 349 year: 2012 end-page: 354 ident: bib1 article-title: Expression of publication-title: Reprod Contracep (in Chinese) contributor: fullname: Wu – volume: 71 start-page: 334 year: 1999 end-page: 341 ident: bib5 article-title: Cytogenetic diagnosis of “normal 46,XX” karyotypes in spontaneous abortions frequently may be misleading publication-title: Fertil Steril contributor: fullname: Haddad – start-page: 269 year: 1989 ident: bib3 publication-title: Chromosomal Disorder contributor: fullname: Li |
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Snippet | Objective To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. Methods A total... To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. A total of 201 cases were... |
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SubjectTerms | early pregnancy fluorescence in situ hybridization (FISH) karyotype analysis spontaneous abortion 异常核型 杂交分析 染色体异常 染色体核型分析 病因 自然流产 荧光原位杂交 诊断 |
Title | Comparative Study of Fluorescence in situ Hybridization Analysis and Karyotype Analysis in Spontaneous Abortion Etiology Diagnosis |
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