Green synthesis of 99mTc-labeled-Fe3O4 nanoparticles using Quince seeds extract and evaluation of their cytotoxicity and biodistribution in rats

• Published in: Journal of molecular structure Vol. 1196; pp. 394 - 402
• Main Authors: Rahmani, Reyhaneh, Gharanfoli, Mohsen, Gholamin, Mehran, Darroudi, Majid, Chamani, Jamshidkhan, Sadri, Kayvan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.11.2019
• Subjects: Cytotoxicity; Iron oxide nanoparticles; Magnetic properties; MTT assay; Superparamagnetic; Tc-99 m; Cytotoxicity; Superparamagnetic; Iron oxide nanoparticles; MTT assay; Magnetic properties; Tc-99 m
• DOI: 10.1016/j.molstruc.2019.06.076
• ISSN: 0022-2860

Superparamagnetic iron oxide nanoparticles (SPIONs), with sizes in low ranges, have been satisfactorily synthesized through an environmental green approach that is introduced in this study. We have dealt with the green synthesis of SPIONs by the utilization of Quince (Cydonia oblonga Miller) seeds extract in the role of a bio-stabilizer. For the purpose of obtaining more data on the morphology and size of Cydonia oblonga seed extract/SPIONs, we have performed characterization through the means of field emission scanning microscopy (FESEM) analysis and in accordance with the results, the magnetite nanoparticles have contained a spherical shape and prepared to be well-defined with less than 50 nm, which had been measured by the usage of FESEM and Dynamic light scattering (DLS) techniques while the purity had been based on the characteristic X-ray diffraction (XRD) planes. The saturation magnetization of magnetite nanoparticles, through the utilization of Vibrating Sample Magnetometer (VSM), has been determined to be ∼53 emu/g. Next to being employed as a chemical analysis procedure, Fourier transform infrared (FTIR) spectroscopy has been applied to substantiate the magnetite construction. It has been displayed through the obtained FTIR spectra that the absorption bands have appeared around 637, 580.4, and 440.9 cm−1, which had been in correlation with the available characteristic absorption bands of Fe3O4. The in vitro cytotoxicity studies on A549 cells has mentioned low toxic and non-toxic SPIONs, respectively in low ranges of concentrations (100 μg/ml), as well as being labeled with the application of 99mTcpertechnetate (Tc-99 m) for bio distribution in regards to the in vivo conditions, thus, it is believed that the synthesized SPIONs can help in a variety of medical applications. [Display omitted] •Simple process for the green synthesis of SPIONs using Quince seeds extract.•It was observed that the mean size of spherical shape SPIONs is less than 50 nm.•Synthesized SPIONs are non-toxic and biocompatible for biomedical applications.•99mTc-labeled-Fe3O4 ensures high sensibility in detection of SPIONs in tissue.