Characterization of the single-stranded DNA binding protein pVVGJΦ of VGJΦ phage from Vibrio cholerae

• Published in: Biochimica et biophysica acta. Proteins and proteomics Vol. 1814; no. 9; pp. 1107 - 1112
• Main Authors: Falero, Alina, Caballero, Andy, Trigueros, Sonia, Pérez, Celso, Campos, Javier, Marrero, Karen, Fando, Rafael
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2011
• Subjects: bacteriophages; bioactive properties; bioinformatics; DNA binding protein; DNA-binding proteins; frozen storage; gel chromatography; gel electrophoresis; isoelectric point; molecular weight; pVVGJΦ protein; single-stranded DNA; sodium chloride; sodium phosphate; temperature; Thermostability; VGJΦ phage; Vibrio cholerae; Thermostability; VGJΦ phage; DNA binding protein; pVVGJΦ protein

pVVGJΦ, a single-stranded DNA binding protein of the vibriophage VGJΦ was subject to biochemical analysis. Here, we show that this protein has a general affinity for single-stranded DNA (ssDNA) as documented by Electrophoretic Mobility Shift Assay (EMSA). The apparent molecular weight of the monomer...