Detection of antimitochondrial antibody by dot-immunobinding assay in patients with primary biliary cirrhosis who tested negative by immunofluorescent staining
Detection of antimitochondrial antibody (AMA) is useful for diagnosis of primary biliary cirrhosis (PBC). Although immunofluorescent techniques are widely used for the detection of AMA, their sensitivity and specificity are not sufficient. We developed a dot-immunobinding assay for AMA using aspirat...
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| Published in | Kanzo Vol. 30; no. 2; pp. 189 - 194 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
The Japan Society of Hepatology
1989
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.30.189 |
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| Abstract | Detection of antimitochondrial antibody (AMA) is useful for diagnosis of primary biliary cirrhosis (PBC). Although immunofluorescent techniques are widely used for the detection of AMA, their sensitivity and specificity are not sufficient. We developed a dot-immunobinding assay for AMA using aspirating microplate. This assay method is as sensitive and specific as the immunoblot method. Using this dot-immunobinding method, we observed six patients who tested negetive results for AMA by immunofluorescent staining. The sera of all patients tested positive by the dot-immunobinding assay with varied titer. The clinical course could be followed in three cases and positive results for AMA continued during observation, even though immunofluorescent results became negative. These results suggest that the dot-immunobinding assay is a sensitive and specific technique detecting AMA in PBC. |
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| AbstractList | Detection of antimitochondrial antibody (AMA) is useful for diagnosis of primary biliary cirrhosis (PBC). Although immunofluorescent techniques are widely used for the detection of AMA, their sensitivity and specificity are not sufficient. We developed a dot-immunobinding assay for AMA using aspirating microplate. This assay method is as sensitive and specific as the immunoblot method. Using this dot-immunobinding method, we observed six patients who tested negetive results for AMA by immunofluorescent staining. The sera of all patients tested positive by the dot-immunobinding assay with varied titer. The clinical course could be followed in three cases and positive results for AMA continued during observation, even though immunofluorescent results became negative. These results suggest that the dot-immunobinding assay is a sensitive and specific technique detecting AMA in PBC. |
| Author | SAKAKIBARA, Yuji KITAMI, Noriyuki USUI, Koh ISHII, Hideo |
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| References | 1) Ishii H, Saifuku K, Namihisa T: Multiplicity of mitochondrial inner membrane antigens from beef heart reacting with antimitochondrial antibodies in sera of patients with primary biliary cirrhosis. Immunol Lett 9: 325-330, 1985 10) Klatskin G, Kantor FS: Mitochondrial antibody in primary biliary cirrhosis and other diseases. Ann Intern Med 77: 533-541, 1972 6) 佐々木博:原発性胆汁性肝硬変(PBC)診断基準.厚生省特定疾患難治性の肝炎調査研究班,昭和56年度研究報告, 152, 1981 15) 浪久利彦,石井秀夫,榊原裕司:抗ミトコンドリア抗体(AMA)のDot-immunobinding Assayによる定量的検出法.厚生省特定疾患難治性の肝炎調査研究班,昭和62年度研究報告,印刷中 3) 浪久利彦,石井秀夫,北見啓之,他:AMA対応抗原の経過による変化.厚生省特定疾患難治性の肝炎調査研究班,昭和61年度研究報告, 190-195, 1987. 7) 佐々木博,市田文弘,山本祐夫,他:原発性胆汁性肝硬変全国調査集計結果.厚生省特定疾患難治性の肝炎調査研究班,昭和55年度研究報告, 128-136, 1981 9) Gupta RC: Specificities and characteristics of mitochondrial protein antigens assesed by enzyme-linked immunosorbent assay for antimitochondrial antibodies. Relationship to immunofluorescent and precipitating antibodies. Clin Exp Immunol 59: 604-612, 1985 11) Sasaki H, Inoue K, Higuchi K, et al: Primary biliary cirrhosis in Japan: National survey by the subcommittee on autoimmune hepatitis. Gastroenterol Jpn 20: 476-485, 1985 8) 佐々木博,市田文弘,山本祐夫,他:原発性胆汁性肝硬変全国調査集計結果(第5報).厚生省特定疾患難治性の肝炎調査研究班,昭和59年度研究報告,16-25, 1985 13) Mitchison HC, Bassendine MF, Handrick A, et al: Positive antimitochondrial antibody but normal alkaline phosphatase: Is this primary biliary cirrhosis?. Hepatology 6: 1279-1284, 1986 18) Kaplan MM, Gondolfo JV, Quaroni EG: An enzyme-linked immunosorbent assay (ELISA) for detecting antimitochondrial antibody. Hepatology 4: 727-730, 1984 19) Mouritsen S, Hoier-Madsen M, Demant EJF, et al: Enzyme-linked immunosorbent assay for determination of anti-mitochondrial antibodies. Acta Pathol Microbiol Immunol Scand (C) 93: 205-210, 1985 5) Penner E, Goldenberg H, Meryn S, et al: A dot-immunobinding assay for antimitochondrial antibodies. Hepatology 6: 381-384, 1986 14) 佐々木博,樋口清博,中野護,他:原発性胆汁性肝硬変の周辺疾患-とくにAMA陰性例,Overlapping syndromeを中心に-.肝胆膵 7: 237-243, 1983 12) Munoz LE, Thomas HC, Scheuer PJ, et al: Is mitochondrial antibody diagnostic of primary biliary cirrhosis?. Gut 22: 136-140, 1981 17) Nagai S, Manns M, Meyerzum Bueschenfelde KH, et al: Detection of mitochondrial antibodies directed against the primary biliary cirrhosis (M2) antigen by enzyme-linked immunosorbent assay (ELISA). J Immunol Methods 60: 77-88, 1983 2) Ishii H, Saifuku K, Namihisa T: Reactivities and clinical relevance of antimitochondrial antibodies to four mitochondrial inner membrane proteins in sera of patients with primary biliary cirrhosis. Hepatology 7: 134-136, 1987 4) Towbin H, Gordon J: Immunoblotting and dot immunobinding-Current status and outlook. J Immunol Methods 72: 313-340, 1984 16) 石井秀夫,榊原裕司,臼井康,他:抗ミトコンドリア抗体のDot-immunobinding assayによる定量的検出法.肝臓 29: 823, 1988 |
| References_xml | – reference: 10) Klatskin G, Kantor FS: Mitochondrial antibody in primary biliary cirrhosis and other diseases. Ann Intern Med 77: 533-541, 1972 – reference: 16) 石井秀夫,榊原裕司,臼井康,他:抗ミトコンドリア抗体のDot-immunobinding assayによる定量的検出法.肝臓 29: 823, 1988 – reference: 8) 佐々木博,市田文弘,山本祐夫,他:原発性胆汁性肝硬変全国調査集計結果(第5報).厚生省特定疾患難治性の肝炎調査研究班,昭和59年度研究報告,16-25, 1985 – reference: 3) 浪久利彦,石井秀夫,北見啓之,他:AMA対応抗原の経過による変化.厚生省特定疾患難治性の肝炎調査研究班,昭和61年度研究報告, 190-195, 1987. – reference: 2) Ishii H, Saifuku K, Namihisa T: Reactivities and clinical relevance of antimitochondrial antibodies to four mitochondrial inner membrane proteins in sera of patients with primary biliary cirrhosis. Hepatology 7: 134-136, 1987 – reference: 14) 佐々木博,樋口清博,中野護,他:原発性胆汁性肝硬変の周辺疾患-とくにAMA陰性例,Overlapping syndromeを中心に-.肝胆膵 7: 237-243, 1983 – reference: 4) Towbin H, Gordon J: Immunoblotting and dot immunobinding-Current status and outlook. J Immunol Methods 72: 313-340, 1984 – reference: 5) Penner E, Goldenberg H, Meryn S, et al: A dot-immunobinding assay for antimitochondrial antibodies. Hepatology 6: 381-384, 1986 – reference: 1) Ishii H, Saifuku K, Namihisa T: Multiplicity of mitochondrial inner membrane antigens from beef heart reacting with antimitochondrial antibodies in sera of patients with primary biliary cirrhosis. Immunol Lett 9: 325-330, 1985 – reference: 17) Nagai S, Manns M, Meyerzum Bueschenfelde KH, et al: Detection of mitochondrial antibodies directed against the primary biliary cirrhosis (M2) antigen by enzyme-linked immunosorbent assay (ELISA). J Immunol Methods 60: 77-88, 1983 – reference: 19) Mouritsen S, Hoier-Madsen M, Demant EJF, et al: Enzyme-linked immunosorbent assay for determination of anti-mitochondrial antibodies. Acta Pathol Microbiol Immunol Scand (C) 93: 205-210, 1985 – reference: 12) Munoz LE, Thomas HC, Scheuer PJ, et al: Is mitochondrial antibody diagnostic of primary biliary cirrhosis?. Gut 22: 136-140, 1981 – reference: 9) Gupta RC: Specificities and characteristics of mitochondrial protein antigens assesed by enzyme-linked immunosorbent assay for antimitochondrial antibodies. Relationship to immunofluorescent and precipitating antibodies. Clin Exp Immunol 59: 604-612, 1985 – reference: 18) Kaplan MM, Gondolfo JV, Quaroni EG: An enzyme-linked immunosorbent assay (ELISA) for detecting antimitochondrial antibody. Hepatology 4: 727-730, 1984 – reference: 15) 浪久利彦,石井秀夫,榊原裕司:抗ミトコンドリア抗体(AMA)のDot-immunobinding Assayによる定量的検出法.厚生省特定疾患難治性の肝炎調査研究班,昭和62年度研究報告,印刷中 – reference: 11) Sasaki H, Inoue K, Higuchi K, et al: Primary biliary cirrhosis in Japan: National survey by the subcommittee on autoimmune hepatitis. Gastroenterol Jpn 20: 476-485, 1985 – reference: 13) Mitchison HC, Bassendine MF, Handrick A, et al: Positive antimitochondrial antibody but normal alkaline phosphatase: Is this primary biliary cirrhosis?. Hepatology 6: 1279-1284, 1986 – reference: 6) 佐々木博:原発性胆汁性肝硬変(PBC)診断基準.厚生省特定疾患難治性の肝炎調査研究班,昭和56年度研究報告, 152, 1981 – reference: 7) 佐々木博,市田文弘,山本祐夫,他:原発性胆汁性肝硬変全国調査集計結果.厚生省特定疾患難治性の肝炎調査研究班,昭和55年度研究報告, 128-136, 1981 |
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| Title | Detection of antimitochondrial antibody by dot-immunobinding assay in patients with primary biliary cirrhosis who tested negative by immunofluorescent staining |
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