Change in pH of Oozed fluid in Periodontal Pocket with Repeated Measurements

oozed fluid in periodontal pocket are influenced by the condition of the surrounding tissues. It might be considered that the surrounding tissues would ooze fluid in reaction to the mechanical stimulation. However, no investigation concerning this reaction has been reported. The aim of this study wa...

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Published inNihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 36; no. 4; pp. 895 - 901
Main Authors KOBAYASHI, Ken'ich, SOEDA, Wataru, NAGAO, Masanori, WATANABE, Tatsutomi
Format Journal Article
LanguageJapanese
Published JAPANESE SOCIETY OF PERIODONTOLOGY 1994
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ISSN0385-0110
1880-408X
DOI10.2329/perio.36.895

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Abstract oozed fluid in periodontal pocket are influenced by the condition of the surrounding tissues. It might be considered that the surrounding tissues would ooze fluid in reaction to the mechanical stimulation. However, no investigation concerning this reaction has been reported. The aim of this study was to clarify the reaction of surrounded tissues by performing repeated measurements of the gingival fluid pH. The values of pH in periodontal pockets of the upper premolars were measured repeatedly. Participants in this study were 10 persons. The values of pH were measured with METOXY. The sensor was made by D. C. reactive sputtering of iridium oxide on the solid substrates, and was 0.5mm in diameter, and 10mm in length. The time needed for measurement was about 5 second with this sensor. The following result were obtained: The pH value changed between the second-third measurement in nine subjects. Eight persons inclined to the alkaline side, one to the acid side and one showed no change. This result indicated that tissues surrounding periodontal pocket reacts against stimulation caused by measurements and oozed reactive fluid. As the sensor in this study was small and the measurement time was short, the mechanical stimulation caused by measurements could be considered less than previous reports. In spite of this, it is meaningful that the tissue surrounding the gingival pocket showed a reaction against this small stimulation. Our result indicates that special care is needed in studying periodontal pocket fluid, and the repeated measurements are not recommended.
AbstractList oozed fluid in periodontal pocket are influenced by the condition of the surrounding tissues. It might be considered that the surrounding tissues would ooze fluid in reaction to the mechanical stimulation. However, no investigation concerning this reaction has been reported. The aim of this study was to clarify the reaction of surrounded tissues by performing repeated measurements of the gingival fluid pH. The values of pH in periodontal pockets of the upper premolars were measured repeatedly. Participants in this study were 10 persons. The values of pH were measured with METOXY. The sensor was made by D. C. reactive sputtering of iridium oxide on the solid substrates, and was 0.5mm in diameter, and 10mm in length. The time needed for measurement was about 5 second with this sensor. The following result were obtained: The pH value changed between the second-third measurement in nine subjects. Eight persons inclined to the alkaline side, one to the acid side and one showed no change. This result indicated that tissues surrounding periodontal pocket reacts against stimulation caused by measurements and oozed reactive fluid. As the sensor in this study was small and the measurement time was short, the mechanical stimulation caused by measurements could be considered less than previous reports. In spite of this, it is meaningful that the tissue surrounding the gingival pocket showed a reaction against this small stimulation. Our result indicates that special care is needed in studying periodontal pocket fluid, and the repeated measurements are not recommended.
Author WATANABE, Tatsutomi
KOBAYASHI, Ken'ich
NAGAO, Masanori
SOEDA, Wataru
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  fullname: SOEDA, Wataru
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  fullname: NAGAO, Masanori
  organization: Tokyo Medical and Dental University, Faculty of Dentistry, Department of Geriatric Dentistry
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  fullname: WATANABE, Tatsutomi
  organization: Tokyo Medical and Dental University, Faculty of Dentistry, Department of Geriatric Dentistry
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References Biswas, S., Duperon, D. F. and Chebib, F. S.: Study of periodontal disease in children and young adolescents. J. Perio. Res., 12: 250-264, 1977.
藤川謙次, 二沼崎光, 小林雅実, 菅野直之, 戸田真一, 村井正大: 歯周ポケット滲出液中のpHに関する研究-pHメーターの検討およびその測定値と臨床所見, 細菌叢との関係-. 日歯周誌, 31: 241-248, 1989.
渡邊竜登美, 小林賢一, 長尾正憲: 歯肉溝内pH計測用センサ. 医用電子と生体工学, 32特別号, 266, 1994.
Bibby, B. G., Mundorff, S. A., Zero, D. A. and Almekinder, K. J.: Oral food clearance and the pH of plaque and saliva. J. A. D. A., 112: 333-337, 1986.
Kato, A., Konno, Y., Yanagida, Y., Yamasato, M., Taguchi, T. and Motohashi, R.: “METOX pH SENSOR” as pH measuring electrode. Analytical Science, 7: 1577-1580, 1991.
添田弥, 渡邊竜登美, 小林賢一, 長尾正憲, 末田武: ポケット探針型pHセンサーの開発. 日歯周誌, 35春期特別号, 137, 1993.
Kleinberg, I.: The construction and evaluation of modified types of antimony micro-electrodes for intra-oral use. British. Dent. J., 104: 197-204, 1958.
Borden, S. M., Golub, L. M. and Kleinberg, I.: The effect of age and sex on the relationship between crevicular fluid flow and gingival inflammation in humans. J. Perio. Res., 6: 160-165, 1977.
Golub, L. M., Borden, S. M. and Kleinberg, I. : Urea content of gingival crevicular fluid and its relation to periodontal disease in humans. J. Perio. Res., 6: 243-251, 1971.
Bickel, W. and Cimasoni, G.: The pH of human crevicular fluid measured by a new microanalytical technique. J. Perio. Res., 20: 35-40, 1985.
片山繁樹, 二家本晃, 陣内純, 尾側雅彦, 芝〓彦: 支台歯における歯周組織の評価第2報歯内滲出液量とpHの変動について. 補綴誌, 31: 139-143, 1987.
沼崎光: 歯周初期治療が歯周ポケット滲出液pHに及ぼす影響について. 日歯周誌, 33: 261-269, 1991.
片山繁樹, 河江信, 芝〓彦: 支台歯における歯周組織の評価第1報歯肉溝および歯周ポケット内のpH値について. 補綴誌, 30: 665-670, 1986.
五十嵐公英: 特殊微小pH電極による砂糖溶液摂取後の口腔内歯垢下pH変化の測定. 小児歯科学雑誌, 18: 385-392, 1980.
Kobayashi, K. Watanabe. T. Soeda, W. Nagao and M. Kato, A.: Clinical Application of Pocket probe Type pH Sensor. I. A. D. R., 73: 117, 1994
Yamada, T., Igarashi, K. and Mitsutomi, M.: Evaluation of cariogenicity of glycosylsucrose by a new method to measure pH under human dental plaque in situ, J. Dent. Res, 59: 2157-2162, 1980.
遠藤英雄: ヒト歯肉溝およびポケット内のpHとPO2. 日歯周誌, 25: 688-708, 1983.
Biswas, S., Duperon, D. F. and Chebib, F. S.: Study of crevice fluid in relation to periodontal disease in children. J. Perio. Res., 12: 265-278, 1977.
References_xml – reference: Borden, S. M., Golub, L. M. and Kleinberg, I.: The effect of age and sex on the relationship between crevicular fluid flow and gingival inflammation in humans. J. Perio. Res., 6: 160-165, 1977.
– reference: Kobayashi, K. Watanabe. T. Soeda, W. Nagao and M. Kato, A.: Clinical Application of Pocket probe Type pH Sensor. I. A. D. R., 73: 117, 1994
– reference: 五十嵐公英: 特殊微小pH電極による砂糖溶液摂取後の口腔内歯垢下pH変化の測定. 小児歯科学雑誌, 18: 385-392, 1980.
– reference: 添田弥, 渡邊竜登美, 小林賢一, 長尾正憲, 末田武: ポケット探針型pHセンサーの開発. 日歯周誌, 35春期特別号, 137, 1993.
– reference: Kleinberg, I.: The construction and evaluation of modified types of antimony micro-electrodes for intra-oral use. British. Dent. J., 104: 197-204, 1958.
– reference: 片山繁樹, 二家本晃, 陣内純, 尾側雅彦, 芝〓彦: 支台歯における歯周組織の評価第2報歯内滲出液量とpHの変動について. 補綴誌, 31: 139-143, 1987.
– reference: Kato, A., Konno, Y., Yanagida, Y., Yamasato, M., Taguchi, T. and Motohashi, R.: “METOX pH SENSOR” as pH measuring electrode. Analytical Science, 7: 1577-1580, 1991.
– reference: Bickel, W. and Cimasoni, G.: The pH of human crevicular fluid measured by a new microanalytical technique. J. Perio. Res., 20: 35-40, 1985.
– reference: 沼崎光: 歯周初期治療が歯周ポケット滲出液pHに及ぼす影響について. 日歯周誌, 33: 261-269, 1991.
– reference: 渡邊竜登美, 小林賢一, 長尾正憲: 歯肉溝内pH計測用センサ. 医用電子と生体工学, 32特別号, 266, 1994.
– reference: Biswas, S., Duperon, D. F. and Chebib, F. S.: Study of crevice fluid in relation to periodontal disease in children. J. Perio. Res., 12: 265-278, 1977.
– reference: 遠藤英雄: ヒト歯肉溝およびポケット内のpHとPO2. 日歯周誌, 25: 688-708, 1983.
– reference: 藤川謙次, 二沼崎光, 小林雅実, 菅野直之, 戸田真一, 村井正大: 歯周ポケット滲出液中のpHに関する研究-pHメーターの検討およびその測定値と臨床所見, 細菌叢との関係-. 日歯周誌, 31: 241-248, 1989.
– reference: Golub, L. M., Borden, S. M. and Kleinberg, I. : Urea content of gingival crevicular fluid and its relation to periodontal disease in humans. J. Perio. Res., 6: 243-251, 1971.
– reference: Biswas, S., Duperon, D. F. and Chebib, F. S.: Study of periodontal disease in children and young adolescents. J. Perio. Res., 12: 250-264, 1977.
– reference: 片山繁樹, 河江信, 芝〓彦: 支台歯における歯周組織の評価第1報歯肉溝および歯周ポケット内のpH値について. 補綴誌, 30: 665-670, 1986.
– reference: Yamada, T., Igarashi, K. and Mitsutomi, M.: Evaluation of cariogenicity of glycosylsucrose by a new method to measure pH under human dental plaque in situ, J. Dent. Res, 59: 2157-2162, 1980.
– reference: Bibby, B. G., Mundorff, S. A., Zero, D. A. and Almekinder, K. J.: Oral food clearance and the pH of plaque and saliva. J. A. D. A., 112: 333-337, 1986.
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Snippet oozed fluid in periodontal pocket are influenced by the condition of the surrounding tissues. It might be considered that the surrounding tissues would ooze...
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SubjectTerms Periodontal pockets
pH
Reacted fluid
Repeated measurements
Title Change in pH of Oozed fluid in Periodontal Pocket with Repeated Measurements
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