Direct activation of protein phosphatase-2A 0 by HIV-1 encoded protein complex NCp7:vpr
The effects of HIV-1 encoded proteins NCp7, vpr and NCp7:vpr complex on the activity of protein phosphatase-2A 0 have been tested. We report that NCp7 is an activator of protein phosphatase-2A 0 and that vpr activated protein phosphatase-2A 0 only slightly. We also report that NCp7 and vpr form a ti...
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Published in | FEBS letters Vol. 401; no. 2; pp. 197 - 201 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
20.01.1997
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Subjects | |
Online Access | Get full text |
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Summary: | The effects of HIV-1 encoded proteins NCp7, vpr and NCp7:vpr complex on the activity of protein phosphatase-2A
0 have been tested. We report that NCp7 is an activator of protein phosphatase-2A
0 and that vpr activated protein phosphatase-2A
0 only slightly. We also report that NCp7 and vpr form a tight complex which becomes a more potent activator of protein phosphatase-2A
0 than NCp7 alone. The ability of NCp7 to activate protein phosphatase-2A
0 is regulated by vpr. The C-terminal portion of vpr prevents NCp7 from activating protein phosphatase-2A
0 while the N-terminal portion of vpr potentiates the effect of NCp7 on the activity of protein phosphatase-2A
0. Our findings indicate that vpr may be acting as a targeting subunit which directs NCp7 to activate protein phosphatase-2A
0. In view of the fact that protein phosphatase-2A functions as an inhibitor of G
2 to M transition of the cell cycle and is involved in other key cellular processes such as the control of RNA transcription, the results presented in this report may explain how HIV-1 causes cell cycle arrest which may lead to CD
4+ T cell depletion and also how it disturbs normal cellular processes of its host cell. |
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ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1016/S0014-5793(96)01470-6 |