Surface tracking integrated extended depth-of-field microscopy for rapid non-contact examination of conjunctival goblet cells in humans
Conjunctival goblet cells (CGCs) are specialized epithelial cells playing key roles for ocular surface homeostasis, and their examination is important for diagnosing ocular surface diseases. Despite recent advancements in high-contrast CGC imaging for non-invasive examination, significant challenges...
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Published in | Biosensors & bioelectronics Vol. 267; p. 116681 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Elsevier B.V
01.01.2025
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Abstract | Conjunctival goblet cells (CGCs) are specialized epithelial cells playing key roles for ocular surface homeostasis, and their examination is important for diagnosing ocular surface diseases. Despite recent advancements in high-contrast CGC imaging for non-invasive examination, significant challenges remain for human applications. High-speed large-area imaging over the curved ocular surface is needed to assess statistically meaningful CGCs in the extensive human conjunctiva. To address this challenge, we developed a novel surface detection method and an integrated microscopy system for human use. With both a long detection range of 2 mm and a high update rate of 50 Hz, the surface detection method enabled real-time surface tracking during large-area imaging. The integrated microscopy could complete 5 × 2 patch imaging in approximately 10 s. CGC density analysis showed significantly reduced uncertainties with large-area imaging. This is the first demonstration of non-contact large-area cellular examination in humans, and this new development holds promise for non-invasive CGC examination and accurate diagnosis of ocular surface diseases. |
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AbstractList | Conjunctival goblet cells (CGCs) are specialized epithelial cells playing key roles for ocular surface homeostasis, and their examination is important for diagnosing ocular surface diseases. Despite recent advancements in high-contrast CGC imaging for non-invasive examination, significant challenges remain for human applications. High-speed large-area imaging over the curved ocular surface is needed to assess statistically meaningful CGCs in the extensive human conjunctiva. To address this challenge, we developed a novel surface detection method and an integrated microscopy system for human use. With both a long detection range of 2 mm and a high update rate of 50 Hz, the surface detection method enabled real-time surface tracking during large-area imaging. The integrated microscopy could complete 5 × 2 patch imaging in approximately 10 s. CGC density analysis showed significantly reduced uncertainties with large-area imaging. This is the first demonstration of non-contact large-area cellular examination in humans, and this new development holds promise for non-invasive CGC examination and accurate diagnosis of ocular surface diseases. Conjunctival goblet cells (CGCs) are specialized epithelial cells playing key roles for ocular surface homeostasis, and their examination is important for diagnosing ocular surface diseases. Despite recent advancements in high-contrast CGC imaging for non-invasive examination, significant challenges remain for human applications. High-speed large-area imaging over the curved ocular surface is needed to assess statistically meaningful CGCs in the extensive human conjunctiva. To address this challenge, we developed a novel surface detection method and an integrated microscopy system for human use. With both a long detection range of 2 mm and a high update rate of 50 Hz, the surface detection method enabled real-time surface tracking during large-area imaging. The integrated microscopy could complete 5 × 2 patch imaging in approximately 10 s. CGC density analysis showed significantly reduced uncertainties with large-area imaging. This is the first demonstration of non-contact large-area cellular examination in humans, and this new development holds promise for non-invasive CGC examination and accurate diagnosis of ocular surface diseases.Conjunctival goblet cells (CGCs) are specialized epithelial cells playing key roles for ocular surface homeostasis, and their examination is important for diagnosing ocular surface diseases. Despite recent advancements in high-contrast CGC imaging for non-invasive examination, significant challenges remain for human applications. High-speed large-area imaging over the curved ocular surface is needed to assess statistically meaningful CGCs in the extensive human conjunctiva. To address this challenge, we developed a novel surface detection method and an integrated microscopy system for human use. With both a long detection range of 2 mm and a high update rate of 50 Hz, the surface detection method enabled real-time surface tracking during large-area imaging. The integrated microscopy could complete 5 × 2 patch imaging in approximately 10 s. CGC density analysis showed significantly reduced uncertainties with large-area imaging. This is the first demonstration of non-contact large-area cellular examination in humans, and this new development holds promise for non-invasive CGC examination and accurate diagnosis of ocular surface diseases. |
ArticleNumber | 116681 |
Author | Yoon, Chang Ho Kim, Ki Hean Choi, Wan Jae Ryu, Jin Suk Kim, Seonghan Lee, Jungbin |
Author_xml | – sequence: 1 givenname: Jungbin orcidid: 0000-0001-6914-5310 surname: Lee fullname: Lee, Jungbin organization: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 37673, Republic of Korea – sequence: 2 givenname: Seonghan surname: Kim fullname: Kim, Seonghan organization: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 37673, Republic of Korea – sequence: 3 givenname: Wan Jae surname: Choi fullname: Choi, Wan Jae organization: Department of Ophthalmology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea – sequence: 4 givenname: Jin Suk surname: Ryu fullname: Ryu, Jin Suk organization: Department of Ophthalmology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea – sequence: 5 givenname: Chang Ho orcidid: 0000-0003-2203-5709 surname: Yoon fullname: Yoon, Chang Ho email: ifree7@gmail.com organization: Department of Ophthalmology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea – sequence: 6 givenname: Ki Hean orcidid: 0000-0003-3410-7706 surname: Kim fullname: Kim, Ki Hean email: kiheankim@postech.ac.kr organization: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 37673, Republic of Korea |
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Cites_doi | 10.1364/BOE.401896 10.1016/0165-1684(94)90060-4 10.1186/s12886-019-1277-9 10.1016/S0161-6420(97)30330-3 10.1016/j.jtos.2019.12.006 10.1097/01.ico.0000224648.74095.90 10.1038/s41385-022-00551-6 10.1038/s41566-018-0109-4 10.1136/bjo.2009.161059 10.1038/s41467-023-37101-y 10.1117/1.1473639 10.1016/j.preteyeres.2018.11.007 10.1364/OE.16.008670 10.1080/02713680701198742 10.1038/s41592-021-01208-1 10.1364/BOE.6.004353 10.1364/OPTICA.5.000337 10.1001/archopht.1984.01040030851031 10.1038/s41598-018-37186-2 10.1016/j.jtos.2022.03.003 10.1001/archopht.120.3.330 10.1016/j.jtos.2012.05.001 10.1016/j.jtos.2019.11.005 10.1111/vop.12343 10.1038/nmeth.2277 10.1097/ICO.0b013e31820ca468 10.1364/OE.25.027770 10.1364/OPTICA.6.000794 10.1021/acsphotonics.0c01774 10.1001/jamaophthalmol.2017.2019 10.1109/TMI.2022.3151944 10.1093/bioinformatics/btx180 10.1364/OL.45.000260 10.3109/02713683.2015.1133832 |
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Keywords | Non-invasive conjunctival goblet cell imaging in humans Real-time surface detection and tracking Extended depth-of-field imaging Large-area non-contact imaging Moxifloxacin-based fluorescence microscopy |
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References | Baudouin, Rolando, Benitez Del Castillo, Messmer, Figueiredo, Irkec, Van Setten, Labetoulle (bib6) 2019; 71 Kim, Kim, K H (bib21) 1992 Lee, Kim, Kim, Son, Yoon, Kim, Kim (bib27) 2022; 41 Wei, Roberts (bib47) 2018; 8 Pertuz (bib37) 2018; 16 Messmer, Mackert, Zapp, Kampik (bib33) 2006; 25 Kunert, Tisdale, Gipson (bib25) 2002; 120 Abrahamsson, Chen, Hajj, Stallinga, Katsov, Wisniewski, Mizuguchi, Soule, Mueller, Darzacq, Darzacq, Wu, Bargmann, Agard, Dahan, Gustafsson (bib1) 2013; 10 Meyer (bib34) 1994; 38 Wang, Lei, Yao, Cai, Liang, Yang, Yang, Li, Xiong (bib46) 2015; 6 Arganda-Carreras, Kaynig, Rueden, Eliceiri, Schindelin, Cardona, Seung (bib4) 2017; 33 Yazdanfar, Kenny, Tasimi, Corwin, Dixon, Filkins (bib50) 2008; 16 Guo, Bian, Jiang, Murphy, Zhu, Wang, Song, Shao, Zhang, Zheng (bib16) 2020; 45 Li (bib28) 2002; 41 Kim, Ahn, Lee, Kim (bib23) 2020; 20 Ren, Xu, Lam (bib42) 2018; 5 Silvestri, Müllenbroich, Costantini, Di Giovanna, Mazzamuto, Franceschini, Kutra, Kreshuk, Checcucci, Toresano, Frasconi, Sacconi, Pavone (bib44) 2021; 18 Wei, Hong, Sun, Xu (bib48) 2011; 30 Doughty (bib13) 2016; 41 Hong, Zhu, Zhuang, Xu, Sun, Le, Li, Wang (bib18) 2010; 94 Descloux, Grußmayer, Bostan, Lukes, Bouwens, Sharipov, Geissbuehler, Mahul-Mellier, Lashuel, Leutenegger, Lasser (bib10) 2018; 12 Matsuzawa, Ando, Fukase, Kimura, Kume, Ide, Izawa, Kaitani, Hara, Nakamura, Kamei, Matsuda, Nakano, Maeda, Tada, Ogawa, Okumura, Murakami, Ebihara, Kitaura (bib32) 2023; 14 Cinotti, Singer, Labeille, Grivet, Rubegni, Douchet, Cambazard, Thuret, Gain, Perrot (bib8) 2017; 135 Altinors, Bozbeyoglu, Karabay, Akova (bib3) 2007; 32 Doughty (bib12) 2012; 10 de Paiva, C.S., St Leger, A.J, Caspi, R.R., 2022. 15, 1143–1157. J D, J C (bib19) 1984; 102 Kim, Lee, Chang, Kim, Kim, Kim (bib22) 2019; 9 Lee, Kim, Yoon, Kim, Kim (bib26) 2020; 11 Pflugfelder, Tseng, Yoshino, Monroy, Felix, Reis (bib38) 1997; 104 Luo, Huang, Rivenson, Ozcan (bib31) 2021; 8 Dunsby (bib14) 2009; 16 Sebbag, Reilly, Eid, Maggs (bib43) 2016; 19 . Hillmann, Spahr, Sudkamp, Hain, Hinkel, Franke, Hüttmann (bib17) 2017; 25 Lu, Hua (bib30) 2015; 23 Alam, de Paiva, Pflugfelder (bib2) 2020; 18 Kim, Jung, Lee, Lee, Ryu, Jang, Myoung, Yang, Chang Ho Yoon (bib24) 2022; 24 Pinkard, Phillips, Babakhani, Fletcher, Waller (bib39) 2019; 6 Swamynathan, Wells (bib45) 2020; 18 Ren (10.1016/j.bios.2024.116681_bib42) 2018; 5 Arganda-Carreras (10.1016/j.bios.2024.116681_bib4) 2017; 33 Li (10.1016/j.bios.2024.116681_bib28) 2002; 41 Luo (10.1016/j.bios.2024.116681_bib31) 2021; 8 Wei (10.1016/j.bios.2024.116681_bib47) 2018; 8 Lu (10.1016/j.bios.2024.116681_bib30) 2015; 23 Guo (10.1016/j.bios.2024.116681_bib16) 2020; 45 Lee (10.1016/j.bios.2024.116681_bib26) 2020; 11 Pflugfelder (10.1016/j.bios.2024.116681_bib38) 1997; 104 Yazdanfar (10.1016/j.bios.2024.116681_bib50) 2008; 16 Sebbag (10.1016/j.bios.2024.116681_bib43) 2016; 19 Pertuz (10.1016/j.bios.2024.116681_bib37) 2018; 16 Wei (10.1016/j.bios.2024.116681_bib48) 2011; 30 J D (10.1016/j.bios.2024.116681_bib19) 1984; 102 Kim (10.1016/j.bios.2024.116681_bib22) 2019; 9 Kunert (10.1016/j.bios.2024.116681_bib25) 2002; 120 Alam (10.1016/j.bios.2024.116681_bib2) 2020; 18 Baudouin (10.1016/j.bios.2024.116681_bib6) 2019; 71 Dunsby (10.1016/j.bios.2024.116681_bib14) 2009; 16 Wang (10.1016/j.bios.2024.116681_bib46) 2015; 6 Abrahamsson (10.1016/j.bios.2024.116681_bib1) 2013; 10 Hong (10.1016/j.bios.2024.116681_bib18) 2010; 94 Meyer (10.1016/j.bios.2024.116681_bib34) 1994; 38 Matsuzawa (10.1016/j.bios.2024.116681_bib32) 2023; 14 Silvestri (10.1016/j.bios.2024.116681_bib44) 2021; 18 Doughty (10.1016/j.bios.2024.116681_bib12) 2012; 10 Doughty (10.1016/j.bios.2024.116681_bib13) 2016; 41 Kim (10.1016/j.bios.2024.116681_bib21) 1992 Kim (10.1016/j.bios.2024.116681_bib24) 2022; 24 Lee (10.1016/j.bios.2024.116681_bib27) 2022; 41 Cinotti (10.1016/j.bios.2024.116681_bib8) 2017; 135 Altinors (10.1016/j.bios.2024.116681_bib3) 2007; 32 Kim (10.1016/j.bios.2024.116681_bib23) 2020; 20 Swamynathan (10.1016/j.bios.2024.116681_bib45) 2020; 18 Hillmann (10.1016/j.bios.2024.116681_bib17) 2017; 25 10.1016/j.bios.2024.116681_bib9 Descloux (10.1016/j.bios.2024.116681_bib10) 2018; 12 Pinkard (10.1016/j.bios.2024.116681_bib39) 2019; 6 Messmer (10.1016/j.bios.2024.116681_bib33) 2006; 25 |
References_xml | – volume: 14 start-page: 1 year: 2023 end-page: 15 ident: bib32 publication-title: Nat. Commun. contributor: fullname: Kitaura – volume: 25 start-page: 27770 year: 2017 end-page: 27784 ident: bib17 publication-title: Opt Express contributor: fullname: Hüttmann – volume: 10 start-page: 149 year: 2012 end-page: 169 ident: bib12 publication-title: Ocul. Surf. contributor: fullname: Doughty – volume: 120 start-page: 330 year: 2002 end-page: 337 ident: bib25 publication-title: Arch. Ophthalmol. contributor: fullname: Gipson – volume: 38 start-page: 113 year: 1994 end-page: 125 ident: bib34 publication-title: Signal Process. contributor: fullname: Meyer – volume: 41 start-page: 1289 year: 2002 ident: bib28 article-title: Autofocus system for microscope publication-title: Opt. Eng. contributor: fullname: Li – volume: 18 start-page: 19 year: 2020 end-page: 26 ident: bib45 publication-title: Ocul. Surf. contributor: fullname: Wells – volume: 6 start-page: 4353 year: 2015 ident: bib46 publication-title: Biomed. Opt Express contributor: fullname: Xiong – volume: 41 start-page: 1393 year: 2016 end-page: 1399 ident: bib13 publication-title: Curr. Eye Res. contributor: fullname: Doughty – volume: 16 start-page: 186 year: 2009 end-page: 196 ident: bib14 publication-title: Opt. InfoBase Conf. Pap. contributor: fullname: Dunsby – volume: 23 year: 2015 ident: bib30 publication-title: Opt Express contributor: fullname: Hua – volume: 5 start-page: 337 year: 2018 ident: bib42 publication-title: Optica contributor: fullname: Lam – volume: 32 start-page: 301 year: 2007 end-page: 307 ident: bib3 publication-title: Curr. Eye Res. contributor: fullname: Akova – volume: 9 start-page: 1 year: 2019 end-page: 7 ident: bib22 publication-title: Sci. Rep. contributor: fullname: Kim – volume: 18 start-page: 953 year: 2021 end-page: 958 ident: bib44 publication-title: Nat. Methods contributor: fullname: Pavone – start-page: 427 year: 1992 end-page: 435 ident: bib21 publication-title: J. Korean Ophthalmol. Soc contributor: fullname: K H – volume: 94 start-page: 1454 year: 2010 end-page: 1458 ident: bib18 publication-title: Br. J. Ophthalmol. contributor: fullname: Wang – volume: 41 start-page: 2004 year: 2022 end-page: 2008 ident: bib27 publication-title: IEEE Trans. Med. Imag. contributor: fullname: Kim – volume: 16 start-page: 3 year: 2018 end-page: 11 ident: bib37 article-title: Shape from focus file exch publication-title: MATLAB Cent. contributor: fullname: Pertuz – volume: 11 start-page: 4890 year: 2020 ident: bib26 publication-title: Biomed. Opt Express contributor: fullname: Kim – volume: 71 start-page: 68 year: 2019 end-page: 87 ident: bib6 publication-title: Prog. Retin. Eye Res. contributor: fullname: Labetoulle – volume: 20 start-page: 1 year: 2020 end-page: 12 ident: bib23 publication-title: BMC Ophthalmol. contributor: fullname: Kim – volume: 12 start-page: 165 year: 2018 end-page: 172 ident: bib10 publication-title: Nat. Photonics contributor: fullname: Lasser – volume: 104 start-page: 223 year: 1997 end-page: 235 ident: bib38 publication-title: Ophthalmology contributor: fullname: Reis – volume: 24 start-page: 100 year: 2022 end-page: 102 ident: bib24 publication-title: Ocul. Surf. contributor: fullname: Chang Ho Yoon – volume: 102 start-page: 1049 year: 1984 end-page: 1051 ident: bib19 article-title: Conjunctival goblet cell densities in ocular surface disease publication-title: Arch. Ophthalmol. contributor: fullname: J C – volume: 8 start-page: 625 year: 2021 end-page: 638 ident: bib31 publication-title: ACS Photonics contributor: fullname: Ozcan – volume: 19 start-page: 38 year: 2016 end-page: 43 ident: bib43 publication-title: Vet. Ophthalmol. contributor: fullname: Maggs – volume: 30 start-page: 1007 year: 2011 end-page: 1012 ident: bib48 publication-title: Cornea contributor: fullname: Xu – volume: 18 start-page: 326 year: 2020 end-page: 334 ident: bib2 publication-title: Ocul. Surf. contributor: fullname: Pflugfelder – volume: 135 start-page: 845 year: 2017 end-page: 851 ident: bib8 publication-title: JAMA Ophthalmol contributor: fullname: Perrot – volume: 10 start-page: 60 year: 2013 end-page: 63 ident: bib1 publication-title: Nat. Methods contributor: fullname: Gustafsson – volume: 8 start-page: 1 year: 2018 end-page: 10 ident: bib47 publication-title: Sci. Rep. contributor: fullname: Roberts – volume: 33 start-page: 2424 year: 2017 end-page: 2426 ident: bib4 publication-title: Bioinformatics contributor: fullname: Seung – volume: 25 start-page: 781 year: 2006 end-page: 788 ident: bib33 publication-title: Cornea contributor: fullname: Kampik – volume: 16 start-page: 8670 year: 2008 ident: bib50 publication-title: Opt Express contributor: fullname: Filkins – volume: 6 start-page: 794 year: 2019 ident: bib39 publication-title: Optica contributor: fullname: Waller – volume: 45 start-page: 260 year: 2020 ident: bib16 publication-title: Opt. Lett. contributor: fullname: Zheng – volume: 11 start-page: 4890 year: 2020 ident: 10.1016/j.bios.2024.116681_bib26 publication-title: Biomed. Opt Express doi: 10.1364/BOE.401896 contributor: fullname: Lee – volume: 38 start-page: 113 year: 1994 ident: 10.1016/j.bios.2024.116681_bib34 publication-title: Signal Process. doi: 10.1016/0165-1684(94)90060-4 contributor: fullname: Meyer – volume: 16 start-page: 186 year: 2009 ident: 10.1016/j.bios.2024.116681_bib14 publication-title: Opt. InfoBase Conf. Pap. contributor: fullname: Dunsby – volume: 20 start-page: 1 year: 2020 ident: 10.1016/j.bios.2024.116681_bib23 publication-title: BMC Ophthalmol. doi: 10.1186/s12886-019-1277-9 contributor: fullname: Kim – volume: 23 year: 2015 ident: 10.1016/j.bios.2024.116681_bib30 publication-title: Opt Express contributor: fullname: Lu – volume: 104 start-page: 223 year: 1997 ident: 10.1016/j.bios.2024.116681_bib38 publication-title: Ophthalmology doi: 10.1016/S0161-6420(97)30330-3 contributor: fullname: Pflugfelder – volume: 18 start-page: 326 year: 2020 ident: 10.1016/j.bios.2024.116681_bib2 publication-title: Ocul. Surf. doi: 10.1016/j.jtos.2019.12.006 contributor: fullname: Alam – volume: 25 start-page: 781 year: 2006 ident: 10.1016/j.bios.2024.116681_bib33 publication-title: Cornea doi: 10.1097/01.ico.0000224648.74095.90 contributor: fullname: Messmer – ident: 10.1016/j.bios.2024.116681_bib9 doi: 10.1038/s41385-022-00551-6 – volume: 12 start-page: 165 year: 2018 ident: 10.1016/j.bios.2024.116681_bib10 publication-title: Nat. Photonics doi: 10.1038/s41566-018-0109-4 contributor: fullname: Descloux – volume: 94 start-page: 1454 year: 2010 ident: 10.1016/j.bios.2024.116681_bib18 publication-title: Br. J. Ophthalmol. doi: 10.1136/bjo.2009.161059 contributor: fullname: Hong – volume: 14 start-page: 1 year: 2023 ident: 10.1016/j.bios.2024.116681_bib32 publication-title: Nat. Commun. doi: 10.1038/s41467-023-37101-y contributor: fullname: Matsuzawa – volume: 41 start-page: 1289 year: 2002 ident: 10.1016/j.bios.2024.116681_bib28 article-title: Autofocus system for microscope publication-title: Opt. Eng. doi: 10.1117/1.1473639 contributor: fullname: Li – volume: 16 start-page: 3 year: 2018 ident: 10.1016/j.bios.2024.116681_bib37 article-title: Shape from focus file exch publication-title: MATLAB Cent. contributor: fullname: Pertuz – volume: 71 start-page: 68 year: 2019 ident: 10.1016/j.bios.2024.116681_bib6 publication-title: Prog. Retin. Eye Res. doi: 10.1016/j.preteyeres.2018.11.007 contributor: fullname: Baudouin – volume: 8 start-page: 1 year: 2018 ident: 10.1016/j.bios.2024.116681_bib47 publication-title: Sci. Rep. contributor: fullname: Wei – volume: 16 start-page: 8670 year: 2008 ident: 10.1016/j.bios.2024.116681_bib50 publication-title: Opt Express doi: 10.1364/OE.16.008670 contributor: fullname: Yazdanfar – volume: 32 start-page: 301 year: 2007 ident: 10.1016/j.bios.2024.116681_bib3 publication-title: Curr. Eye Res. doi: 10.1080/02713680701198742 contributor: fullname: Altinors – volume: 18 start-page: 953 year: 2021 ident: 10.1016/j.bios.2024.116681_bib44 publication-title: Nat. Methods doi: 10.1038/s41592-021-01208-1 contributor: fullname: Silvestri – volume: 6 start-page: 4353 year: 2015 ident: 10.1016/j.bios.2024.116681_bib46 publication-title: Biomed. Opt Express doi: 10.1364/BOE.6.004353 contributor: fullname: Wang – volume: 5 start-page: 337 year: 2018 ident: 10.1016/j.bios.2024.116681_bib42 publication-title: Optica doi: 10.1364/OPTICA.5.000337 contributor: fullname: Ren – volume: 102 start-page: 1049 year: 1984 ident: 10.1016/j.bios.2024.116681_bib19 article-title: Conjunctival goblet cell densities in ocular surface disease publication-title: Arch. Ophthalmol. doi: 10.1001/archopht.1984.01040030851031 contributor: fullname: J D – volume: 9 start-page: 1 year: 2019 ident: 10.1016/j.bios.2024.116681_bib22 publication-title: Sci. Rep. doi: 10.1038/s41598-018-37186-2 contributor: fullname: Kim – volume: 24 start-page: 100 year: 2022 ident: 10.1016/j.bios.2024.116681_bib24 publication-title: Ocul. Surf. doi: 10.1016/j.jtos.2022.03.003 contributor: fullname: Kim – volume: 120 start-page: 330 year: 2002 ident: 10.1016/j.bios.2024.116681_bib25 publication-title: Arch. Ophthalmol. doi: 10.1001/archopht.120.3.330 contributor: fullname: Kunert – start-page: 427 year: 1992 ident: 10.1016/j.bios.2024.116681_bib21 publication-title: J. Korean Ophthalmol. Soc contributor: fullname: Kim – volume: 10 start-page: 149 year: 2012 ident: 10.1016/j.bios.2024.116681_bib12 publication-title: Ocul. Surf. doi: 10.1016/j.jtos.2012.05.001 contributor: fullname: Doughty – volume: 18 start-page: 19 year: 2020 ident: 10.1016/j.bios.2024.116681_bib45 publication-title: Ocul. Surf. doi: 10.1016/j.jtos.2019.11.005 contributor: fullname: Swamynathan – volume: 19 start-page: 38 year: 2016 ident: 10.1016/j.bios.2024.116681_bib43 publication-title: Vet. Ophthalmol. doi: 10.1111/vop.12343 contributor: fullname: Sebbag – volume: 10 start-page: 60 year: 2013 ident: 10.1016/j.bios.2024.116681_bib1 publication-title: Nat. Methods doi: 10.1038/nmeth.2277 contributor: fullname: Abrahamsson – volume: 30 start-page: 1007 year: 2011 ident: 10.1016/j.bios.2024.116681_bib48 publication-title: Cornea doi: 10.1097/ICO.0b013e31820ca468 contributor: fullname: Wei – volume: 25 start-page: 27770 year: 2017 ident: 10.1016/j.bios.2024.116681_bib17 publication-title: Opt Express doi: 10.1364/OE.25.027770 contributor: fullname: Hillmann – volume: 6 start-page: 794 year: 2019 ident: 10.1016/j.bios.2024.116681_bib39 publication-title: Optica doi: 10.1364/OPTICA.6.000794 contributor: fullname: Pinkard – volume: 8 start-page: 625 year: 2021 ident: 10.1016/j.bios.2024.116681_bib31 publication-title: ACS Photonics doi: 10.1021/acsphotonics.0c01774 contributor: fullname: Luo – volume: 135 start-page: 845 year: 2017 ident: 10.1016/j.bios.2024.116681_bib8 publication-title: JAMA Ophthalmol doi: 10.1001/jamaophthalmol.2017.2019 contributor: fullname: Cinotti – volume: 41 start-page: 2004 year: 2022 ident: 10.1016/j.bios.2024.116681_bib27 publication-title: IEEE Trans. Med. Imag. doi: 10.1109/TMI.2022.3151944 contributor: fullname: Lee – volume: 33 start-page: 2424 year: 2017 ident: 10.1016/j.bios.2024.116681_bib4 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btx180 contributor: fullname: Arganda-Carreras – volume: 45 start-page: 260 year: 2020 ident: 10.1016/j.bios.2024.116681_bib16 publication-title: Opt. Lett. doi: 10.1364/OL.45.000260 contributor: fullname: Guo – volume: 41 start-page: 1393 year: 2016 ident: 10.1016/j.bios.2024.116681_bib13 publication-title: Curr. Eye Res. doi: 10.3109/02713683.2015.1133832 contributor: fullname: Doughty |
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SubjectTerms | Extended depth-of-field imaging Large-area non-contact imaging Moxifloxacin-based fluorescence microscopy Non-invasive conjunctival goblet cell imaging in humans Real-time surface detection and tracking |
Title | Surface tracking integrated extended depth-of-field microscopy for rapid non-contact examination of conjunctival goblet cells in humans |
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