Histidine Patch Thioredoxins
A cluster of surface amino acid residues on Escherichia coli thioredoxin were systematically mutated in order to provide the molecule with an ability to chelate metal ions. The combined effect of two histidine mutants, E30H and Q62H, gave thioredoxin the capacity to bind to nickel ions immobilized o...
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Published in | The Journal of biological chemistry Vol. 271; no. 9; pp. 5059 - 5065 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
American Society for Biochemistry and Molecular Biology
01.03.1996
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Online Access | Get full text |
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