High-performance liquid chromatography directly coupled to 19F and 1H NMR for the analysis of mixtures of isomeric ester glucuronide conjugates of trifluoromethylbenzoic acids

• Published in: Journal of Chromatography A Vol. 728; no. 1; pp. 377 - 385
• Main Authors: Sidelmann, Ulla G., Nicholls, Andrew W., Meadows, Peter E., Gilbert, John W., Lindon, John C., Wilson, Ian D., Nicholson, Jeremy K.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.03.1996
• Subjects: Acyl migration reactions; Nuclear magnetic resonance spectroscopy; Trifluoromethylbenzoic acid ester glucuronide; Nuclear magnetic resonance spectroscopy; Acyl migration reactions; Trifluoromethylbenzoic acid ester glucuronide

Acyl migration reactions of drug 1-O-acyl glucuronides are of interest because of their possible role in covalent binding to serum proteins and consequent allergic reactions. This paper describes a new way of investigating the kinetics of acyl migration reactions in a buffer system at pH 7.4, using...