Molecular detection of Helicobacter pylori isolated from biopsies of gastritis patients based on the presence of 16srRNA and VacA S1/S2-UreA virulence genes

• Published in: Tikrit Journal of Pure Science Vol. 29; no. 1; pp. 49 - 57
• Main Authors: Alrubaye, Ahood, Awadh, Halah Abdulkhaliq, Faris, Firas
• Format: Journal Article
• Language: English
• Published: Tikrit University 25.02.2024
• Subjects: gastric cancer; urease gene and chronic gastric; VacS1/S2
• ISSN: 1813-1662; 2415-1726
• DOI: 10.25130/tjps.v29i1.1489

Background: Although most infections with Helicobacter pylori are asymptomatic, some people with this bacterium end up with chronic gastritis, peptic ulcers, or even stomach cancer. The study's objectives wasto detectthe prevalence of Helicobacter pylori by identifying 16sRNA and to determine the virulence genes (ureA and vacAS1/S2) in biopsies of patients with gastritis. Method: The study was conducted between September 2022 and June 2023 at the public hospital and several private clinics in Balad City, Iraq. A total of 50 samples were taken, spanningfrom 15-85 years of age (50 patients). Balad General Hospital and a private upper surgical endoscopy clinic. The specialist doctor (gastroenterologist) took the gastric biopsy samples from the stomach's antrum and corpus during normal endoscopy. The gastric biopsies were used for PCR. Results: The current study includes 50 samples suffering from gastritis. Patients according to age groups 26-34 and 45-54 had the highest frequency, while, 55-64 had the lowest frequency.  Helicobacter Pylori was detected by16SrRNA. According to the result 16SrRNA by, the research found that positive gastric patients was42 (84%) and 8(16%) negative. In addition, this research found that the UreA gene was39 (78%) positive and 11(22%) negative and theVacS1/S2 gene was22 (44%) positive and 28(56%) negative.