Antagonistic activity of dominant bacteria isolated from the rhizosphere of spring barley against phytopathogenic micromycetes

• Published in: PLANT AND SOIL SCIENCE Vol. 12; no. 2; pp. 54 - 59
• Main Authors: Kiroiants, M., Patyka, T., Patyka, Mykola
• Format: Journal Article
• Language: English
• Published: National University of Life and Environmental Sciences of Ukraine 01.06.2021
• Subjects: antagonistic properties; bacillus methylotrophicus 10; phyllobacterium ifriqiyense 1; phytopathogenic micromycetes
• ISSN: 2706-7688; 2706-7696
• DOI: 10.31548/agr2021.02.0054

The purpose of the study was to examine the manifestation of representatives of the bacterial biome that dominate the spring barley rhizosphere, antagonistic activity against phytopathogenic micromycetes. The standard diffuse method of double culture in Petri dishes was used to examine the antagonistic properties of bacterial strains,dominating the rhizosphere. The level of antagonistic activity of microorganisms was evaluated by indicator (%) of inhibition of growth and development of mycelium of micromycetes Fusarium sporotrichioides Sherb. 23.2, Alternaria alternata (Fr.) Keissl. 3.45, Nigrospora oryzae (Berk. & Broome) Petch. 18.77. As a result of the conducted studies, it was identified that bacteria Bacillus methylotrophicus 10 had an inhibitory effect on Fusarium sporotrichioides Sherb. 23.2 ‒ 77,4%, Alternaria alternata (Fr.) Keissl. 3.45 ‒ 66.6% and Nigrospora oryzae (Berk. & Broome) Petch. 18.77 ‒ 86.7%, while bacteria Phyllobacterium ifriqiyense 1 showed activity against phytopathogens by 45.1%, 63.1%, and 65.0%, respectively. Thus, both strains of dominating rhizospheric bacteria have high antagonistic activity against phytopathogenic micromycetes, which gives grounds for their further study