A low‐cost protocol for the optical method of vulnerability curves to calculate P 50

The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity ( ) is lost in stems, using a...

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Published inApplications in plant sciences Vol. 13; no. 2; p. e70004
Main Authors González‐Rebeles, Georgina, Alonso‐Arevalo, Miguel Ángel, López, Eulogio, Méndez‐Alonzo, Rodrigo
Format Journal Article
LanguageEnglish
Published United States John Wiley and Sons Inc 01.03.2025
Subjects
Protocol Note
image processing
xylem tissue
cost‐effective protocols
stem water potential
percentage of embolisms accumulated
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Abstract The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity ( ) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method. Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify in an exotic species ( ) and native species ( ) of the Mediterranean vegetation in Baja California, Mexico. The intra- and interspecific patterns of variation in stem of and were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.
AbstractList The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity (P 50) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.PremiseThe quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity (P 50) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify P 50 in an exotic species (Nicotiana glauca) and native species (Rhus integrifolia) of the Mediterranean vegetation in Baja California, Mexico.Methods and ResultsOur protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify P 50 in an exotic species (Nicotiana glauca) and native species (Rhus integrifolia) of the Mediterranean vegetation in Baja California, Mexico.The intra- and interspecific patterns of variation in stem P 50 of N. glauca and R. integrifolia were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.ConclusionsThe intra- and interspecific patterns of variation in stem P 50 of N. glauca and R. integrifolia were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.
The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity ( ) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method. Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify in an exotic species ( ) and native species ( ) of the Mediterranean vegetation in Baja California, Mexico. The intra- and interspecific patterns of variation in stem of and were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.
Author González‐Rebeles, Georgina
López, Eulogio
Alonso‐Arevalo, Miguel Ángel
Méndez‐Alonzo, Rodrigo
AuthorAffiliation 1 Departamento de Biología de la Conservación, Centro de Investigación Científica y de Educación Superior de Ensenada Carretera Ensenada Tijuana No. 3918, Zona Playitas, C.P. 22860 Ensenada Baja California Mexico
3 Departamento de Electrónica y Telecomunicaciones, Centro de Investigación Científica y de Educación Superior de Ensenada Carretera Ensenada Tijuana No. 3918, Zona Playitas, C.P. 22860 Ensenada Baja California Mexico
2 Departamento del Hombre y su Ambiente Universidad Autónoma Metropolitana–Unidad Xochimilco Calzada del Hueso 1100, Colonia Villa Quietud, Alcaldía Coyoacán, C.P. 04960 Ciudad de México Mexico
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– name: 2 Departamento del Hombre y su Ambiente Universidad Autónoma Metropolitana–Unidad Xochimilco Calzada del Hueso 1100, Colonia Villa Quietud, Alcaldía Coyoacán, C.P. 04960 Ciudad de México Mexico
– name: 3 Departamento de Electrónica y Telecomunicaciones, Centro de Investigación Científica y de Educación Superior de Ensenada Carretera Ensenada Tijuana No. 3918, Zona Playitas, C.P. 22860 Ensenada Baja California Mexico
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Keywords image processing
xylem tissue
cost‐effective protocols
stem water potential
percentage of embolisms accumulated
Language English
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Title A low‐cost protocol for the optical method of vulnerability curves to calculate P 50
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