Active Immunization Study of Colon Cancer Derived 1-8D Peptide in HHD Mice

• Published in: Immune network Vol. 5; no. 3; pp. 157 - 162
• Main Authors: Jung, Hun-Soon, Ahn, In-Sook, Do, Hyung-Ki, Lemonnier, Francois A, Song, Kuk-Hyun, Do, Myoung-Sool
• Format: Journal Article
• Language: English
• Published: 대한면역학회 2005
• Subjects: 면역학; TAA (Tumor-associated antigen); 1-8D gene; Colon cancer; Peptide vaccine; HHD mice; Active immunization
• ISSN: 1598-2629; 2092-6685
• DOI: 10.4110/in.2005.5.3.157

Background: 1-8D gene is a member of human 1-8 interferon inducible gene family and was shown to be overexpressed in fresh colon cancer tissues. Three peptides 1-6, 3-5 and 3-7 derived from human 1-8D gene were shown to have immunogenicity against colon cancer. Methods: To study tumor immunotherapy of three peptides we established an active immunization model using HHD mice. Db / β2 microglobulin (β2 m) null mice transgenic for a chimeric HLA-A2.1/Db β2 m single chain (HHD mice) were challenged with B16/HHD/1-8D tumor cells and were immunized with irradiated peptide-loaded RMA- S/HHD/B7.1 transfectants. In therapy model tumor growth was retarded in HHD mice that were injected with 3-5 peptide-loaded RMA-S/HHD/B7.1. In survival test vaccination with 1-8D-derived peptide protects HHD mice from tumor progression after tumor challenge. Results: These studies show that peptide 3-5 derived from 1-8D gene can be the most effective candidate for the vaccine of immunotherapy against colon cancer and highlight 1-8D gene as putative colon carcinoma associated antigens. Conclusion: We demonstrated that RMA-S/HHD/ B7.1 loaded with 1-8D peptides, especially 3-5, immunization generates potent antitumor immunity against tumor cells in HHD mice and designed active immunization as proper immunotherapeutic protocols. KCI Citation Count: 0