Short‐term in vivo modifications of platelet NADPH oxidase 2 ( NOX 2) and prostaglandin F 2α in HIV‐1 patients on abacavir‐based therapies

• Published in: HIV medicine Vol. 17; no. 10; pp. 774 - 777
• Main Authors: Pastori, D, Esposito, A, Carnevale, R, Bartimoccia, S, Nocella, C, Fantauzzi, A, Pignatelli, P, Violi, F, Mezzaroma, I
• Format: Journal Article
• Language: English
• Published: England 01.11.2016
• Subjects: Adolescent; Adult; Anti-HIV Agents - therapeutic use; Blood Platelets - chemistry; Blood Platelets - enzymology; CD40 Ligand - blood; Dideoxynucleosides - therapeutic use; Dinoprost - analysis; Female; HIV Infections - drug therapy; HIV Infections - pathology; Humans; Male; Membrane Glycoproteins - analysis; Middle Aged; NADPH Oxidase 2; NADPH Oxidases - analysis; Pilot Projects; Platelet Activation; Young Adult; abacavir; HIV-1 infection; isoprostanes; NADPH oxidase; platelet activation
• DOI: 10.1111/hiv.12383
• ISSN: 1464-2662

The aim of the study was to investigate the in vivo effect of abacavir (ABC) on platelet oxidative stress. We performed a randomized pilot study including 39 HIV-1-infected patients, 17 on zidovudine/lamivudine (ZDV/3TC) and 22 on tenofovir/emtricitabine (TDF/FTC). Ten patients on ZDV/3TC and eight patients on TDF/FTC were randomly allocated to switching the nucleoside backbone to ABC/3TC. At baseline and after 6 months, platelet oxidative stress was assessed by platelet NADPH oxidase 2 (NOX2)-derived peptide (sNOX2-dp), a marker of NOX2 activation, and platelet prostaglandin F (8-iso-PGF ). Platelet activation was measured by soluble CD40L (sCD40L). At baseline, no differences between ZDV/3TC or TDF/FTC recipients were found. After 6 months, patients switching from ZDV/3TC showed a decrease of sNOX2-dp (from 20.9±5.7 to 12.5±3.8 pg/ml, p=0.002) and 8-iso-PGF (from 154.3±41.9 to 122.9±28.0 pmol/l, p=0.025). No effects on platelet oxidative stress biomarkers were observed in subjects from TDF/FTC, who showed a significant increase in blood glucose (p=0.043) and total cholesterol (p=0.027). ABC showed no effect on sCD40L levels in both groups. ABC reduced platelet sNOX2-dp and 8-iso-PGF in HIV-1 subjects switching from ZDV/3TC but not in those from TDF/FTC after 6 months. No changes in platelet activation were found in both groups.