Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced byAspergillus caespitosus

• Published in: Folia microbiologica Vol. 52; no. 3; pp. 231 - 236
• Main Authors: Guimarães, L. H. S., Júnior, A. B., Jorge, J. A., Terenzi, H. F., Polizeli, M. L. T. M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.05.2007
• Subjects: Alkaline phosphatase; Biochemistry; Carbohydrates; Cellulose; Concanavalin A; Deoxyribonucleic acid; DNA; Enzymes; Gel electrophoresis; Magnesium; Microbiology; Mycelia; pH effects; Proteins; Recombinant DNA; Sodium lauryl sulfate; Substrates

Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract ofA. caespitosus were described. The enzyme was purified 42-fold with 32 % recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7 % SDS...