Vacuolar-Type ATPase in a Hyperthermophilic Archaeum,Thermococcussp. KI
Membrane ATPase was purified from a hyperthermophilic heterotrophic archaeum,Thermococcussp. KI, which grew anaerobically at 90°C in the presence of sulfur. The purified enzyme had an optimal temperature of 90°C and its molecular mass was estimated to be 600 kDa. It consisted of 4 subunits with mole...
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| Published in | Biochemical and biophysical research communications Vol. 229; no. 2; pp. 559 - 564 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier Inc
13.12.1996
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| Online Access | Get full text |
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| Abstract | Membrane ATPase was purified from a hyperthermophilic heterotrophic archaeum,Thermococcussp. KI, which grew anaerobically at 90°C in the presence of sulfur. The purified enzyme had an optimal temperature of 90°C and its molecular mass was estimated to be 600 kDa. It consisted of 4 subunits with molecular masses of 70, 60, 29 and 15 kDa. While the ATPase activity was resistant to most ATPase inhibitors, the activity was reduced by nitrate, an inhibitor of the vacuolar (V)-type ATPase. N-terminal amino acid sequence of the 70 kDa subunit was similar to those of catalytic subunit of V-type ATPases. This indicates that hyperthermophilic heterotrophs have a V-type ATPase in their membranes. |
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| AbstractList | Membrane ATPase was purified from a hyperthermophilic heterotrophic archaeum,Thermococcussp. KI, which grew anaerobically at 90°C in the presence of sulfur. The purified enzyme had an optimal temperature of 90°C and its molecular mass was estimated to be 600 kDa. It consisted of 4 subunits with molecular masses of 70, 60, 29 and 15 kDa. While the ATPase activity was resistant to most ATPase inhibitors, the activity was reduced by nitrate, an inhibitor of the vacuolar (V)-type ATPase. N-terminal amino acid sequence of the 70 kDa subunit was similar to those of catalytic subunit of V-type ATPases. This indicates that hyperthermophilic heterotrophs have a V-type ATPase in their membranes. |
| Author | Maruyama, Tadashi Kamagata, Yoichi Iida, Toshii Kamino, Kei Inatomi, Ken-ichi Hoaki, Toshihiro |
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| Copyright | 1996 Academic Press |
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| Title | Vacuolar-Type ATPase in a Hyperthermophilic Archaeum,Thermococcussp. KI |
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