吗啡对顺铂诱导的鼻咽癌CNE-2细胞裸鼠 移植瘤生长抑制作用的影响

【目的】 以人鼻咽癌CNE-2细胞裸鼠移植瘤为模型,探讨吗啡对顺铂抑制肿瘤生长作用的影响及其机制65377;【方法】 建立CNE-2细胞裸鼠移植瘤模型20只,随机分为4组,对照组,单纯顺铂用药组65380;单纯吗啡用药组和吗啡联合顺铂用药组,每组5只65377;吗啡5 mg/kg每天给药1次,顺铂3 mg/kg每3 d给药2次,均为腹腔注射65377;用药14 d后处死动物,完整剥离肿瘤,记净瘤质量65377;TUNEL染色计算细胞凋亡数,免疫组化染色检测肿瘤组织Bax,Bcl-2,cleaved-caspase-3以及CD34的表达情况65377;【结果】 单纯顺铂用药组的瘤质量明显小于其它...

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Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 31
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2010
Subjects
CNE-2细胞
凋亡
吗啡
顺铂
Online AccessGet full text
ISSN1672-3554

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Abstract 【目的】 以人鼻咽癌CNE-2细胞裸鼠移植瘤为模型,探讨吗啡对顺铂抑制肿瘤生长作用的影响及其机制65377;【方法】 建立CNE-2细胞裸鼠移植瘤模型20只,随机分为4组,对照组,单纯顺铂用药组65380;单纯吗啡用药组和吗啡联合顺铂用药组,每组5只65377;吗啡5 mg/kg每天给药1次,顺铂3 mg/kg每3 d给药2次,均为腹腔注射65377;用药14 d后处死动物,完整剥离肿瘤,记净瘤质量65377;TUNEL染色计算细胞凋亡数,免疫组化染色检测肿瘤组织Bax,Bcl-2,cleaved-caspase-3以及CD34的表达情况65377;【结果】 单纯顺铂用药组的瘤质量明显小于其它3组(P < 0.05),吗啡联合顺铂用药组的瘤质量明显小于对照组(P < 0.05);TUNEL染色及cleaved-caspase-3染色显示单纯顺铂用药组的凋亡细胞数明显多于其他3组(P < 0.05)65377;单纯顺铂用药组与其他3组相比Bax蛋白表达量增多,而Bcl-2表达量减少,单纯吗啡组的Bcl-2表达最强65377;CD34微血管染色显示单纯顺铂用药组的微血管密度显著少于其它3组(P < 0.05),单纯吗啡用药组的微血管密度显著多于对照组(P < 0.05)65377;【结论】 吗啡通过拮抗顺铂诱导细胞凋亡及促进CNE-2细胞裸鼠移植瘤组织的血管生成,抵抗了顺铂抑制肿瘤生长的作用65377;
AbstractList 【目的】 以人鼻咽癌CNE-2细胞裸鼠移植瘤为模型,探讨吗啡对顺铂抑制肿瘤生长作用的影响及其机制65377;【方法】 建立CNE-2细胞裸鼠移植瘤模型20只,随机分为4组,对照组,单纯顺铂用药组65380;单纯吗啡用药组和吗啡联合顺铂用药组,每组5只65377;吗啡5 mg/kg每天给药1次,顺铂3 mg/kg每3 d给药2次,均为腹腔注射65377;用药14 d后处死动物,完整剥离肿瘤,记净瘤质量65377;TUNEL染色计算细胞凋亡数,免疫组化染色检测肿瘤组织Bax,Bcl-2,cleaved-caspase-3以及CD34的表达情况65377;【结果】 单纯顺铂用药组的瘤质量明显小于其它3组(P < 0.05),吗啡联合顺铂用药组的瘤质量明显小于对照组(P < 0.05);TUNEL染色及cleaved-caspase-3染色显示单纯顺铂用药组的凋亡细胞数明显多于其他3组(P < 0.05)65377;单纯顺铂用药组与其他3组相比Bax蛋白表达量增多,而Bcl-2表达量减少,单纯吗啡组的Bcl-2表达最强65377;CD34微血管染色显示单纯顺铂用药组的微血管密度显著少于其它3组(P < 0.05),单纯吗啡用药组的微血管密度显著多于对照组(P < 0.05)65377;【结论】 吗啡通过拮抗顺铂诱导细胞凋亡及促进CNE-2细胞裸鼠移植瘤组织的血管生成,抵抗了顺铂抑制肿瘤生长的作用65377;
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Snippet 【目的】 以人鼻咽癌CNE-2细胞裸鼠移植瘤为模型,探讨吗啡对顺铂抑制肿瘤生长作用的影响及其机制65377;【方法】 建立CNE-2细胞裸鼠移植瘤模型20只,随机分为4组,对照组,单纯顺铂用药组65380;单纯吗啡用药组和吗啡联合顺铂用药组,每组5只65377;吗啡5 mg/kg每天给药1次,顺铂3 mg/kg每3...
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SubjectTerms CNE-2细胞
凋亡
吗啡
顺铂
Title 吗啡对顺铂诱导的鼻咽癌CNE-2细胞裸鼠 移植瘤生长抑制作用的影响
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