神经生长因子对人视网膜血管内皮细胞的影响

【目的】 观察神经生长因子(NGF)及TrKa阻断剂K252a对体外培养的人视网膜血管内皮细胞(HRCEC)增殖的影响65377; 【方法】 MTT法检测不同因子(NGF各浓度组65380;NGF + K252a各浓度组65380;bFGF组65380;bFGF + K252a组和正常培养液组)对正常和缺氧条件下培养的HRCEC的影响65377; 【结果】 随NGF浓度增加(2065380;5065380;100 ng/ml),HRCEC细胞数目明显增多(正常氧浓度NGF组分别为0.254 ± 0.03365380;0.696 ± 0.02965380;1.136 ± 0.051;缺氧条件相应...

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Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 31
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2010
Subjects
TrkA阻断剂K252a
人视网膜血管内皮细胞(HRCEC)
神经生长因子(NGF)
Online AccessGet full text
ISSN1672-3554

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Abstract 【目的】 观察神经生长因子(NGF)及TrKa阻断剂K252a对体外培养的人视网膜血管内皮细胞(HRCEC)增殖的影响65377; 【方法】 MTT法检测不同因子(NGF各浓度组65380;NGF + K252a各浓度组65380;bFGF组65380;bFGF + K252a组和正常培养液组)对正常和缺氧条件下培养的HRCEC的影响65377; 【结果】 随NGF浓度增加(2065380;5065380;100 ng/ml),HRCEC细胞数目明显增多(正常氧浓度NGF组分别为0.254 ± 0.03365380;0.696 ± 0.02965380;1.136 ± 0.051;缺氧条件相应各组为0.422 ± 0.03665380;0.798 ± 0.04465380;1.376 ± 0.052,P均 < 0.05)65377;随K252a浓度增加(5065380;10065380;200 nmol/L),NGF + K252a组正常氧浓度时分别为0.864 ± 0.06765380;0.496 ± 0.02565380;0.202 ± 0.078,缺氧条件时相应为1.042 ± 0.04765380;0.700 ± 0.06565380;0.401 ± 0.078,较同浓度NGF(100 ng/ml)组HRCEC细胞数目减少(P < 0.05),随K252a浓度增加HRCEC细胞数目减少趋势愈加显著(P均 < 0.05)65377;【结论】 NGF可促进HRCEC的增殖,该作用可被特异性TrkA阻断剂K252a所阻断65377;
AbstractList 【目的】 观察神经生长因子(NGF)及TrKa阻断剂K252a对体外培养的人视网膜血管内皮细胞(HRCEC)增殖的影响65377; 【方法】 MTT法检测不同因子(NGF各浓度组65380;NGF + K252a各浓度组65380;bFGF组65380;bFGF + K252a组和正常培养液组)对正常和缺氧条件下培养的HRCEC的影响65377; 【结果】 随NGF浓度增加(2065380;5065380;100 ng/ml),HRCEC细胞数目明显增多(正常氧浓度NGF组分别为0.254 ± 0.03365380;0.696 ± 0.02965380;1.136 ± 0.051;缺氧条件相应各组为0.422 ± 0.03665380;0.798 ± 0.04465380;1.376 ± 0.052,P均 < 0.05)65377;随K252a浓度增加(5065380;10065380;200 nmol/L),NGF + K252a组正常氧浓度时分别为0.864 ± 0.06765380;0.496 ± 0.02565380;0.202 ± 0.078,缺氧条件时相应为1.042 ± 0.04765380;0.700 ± 0.06565380;0.401 ± 0.078,较同浓度NGF(100 ng/ml)组HRCEC细胞数目减少(P < 0.05),随K252a浓度增加HRCEC细胞数目减少趋势愈加显著(P均 < 0.05)65377;【结论】 NGF可促进HRCEC的增殖,该作用可被特异性TrkA阻断剂K252a所阻断65377;
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Snippet 【目的】 观察神经生长因子(NGF)及TrKa阻断剂K252a对体外培养的人视网膜血管内皮细胞(HRCEC)增殖的影响65377; 【方法】 MTT法检测不同因子(NGF各浓度组65380;NGF + K252a各浓度组65380;bFGF组65380;bFGF +...
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SubjectTerms TrkA阻断剂K252a
人视网膜血管内皮细胞(HRCEC)
神经生长因子(NGF)
Title 神经生长因子对人视网膜血管内皮细胞的影响
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Volume 31
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