Bcl-xl基因对硝普钠诱导SY5Y细胞凋亡的抑制作用

【目的】 研究Bcl-xl基因对成人神经母细胞廇SH-SY5Y细胞毒性的影响.【方法】 将构建好的Bcl-xl基因重组真核表达载体pIRES2-EGFP/Bcl-xl转染SH-SY5Y细胞,并分为正常组、pIRES2-EGFP空质粒转染组、pIRES2-EGFP/Bcl-xl质粒转染组,利用凋亡诱导剂硝普钠(50 μmol/L)诱导各组细胞凋亡,行Hoechst33258细胞染色对比观察各组细胞凋亡情况,通过MTT法分析各组细胞存活率?RT-PCR与Western blotting检测转染后Bcl-xl基因的表达情况.【结果】 转染SH-SY5Y细胞后,检测结果显示Bcl-xl基因能够高效转染...

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Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 33
Main Authors 杨炼红, 蒋龙元, 蔡晓冬, 刘淑琼, 吴秀勤, 陶恩祥
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2012
Subjects
Bcl-xl基因
SH-SY5Y
凋亡
基因治疗
硝普钠
Online AccessGet full text
ISSN1672-3554

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Abstract 【目的】 研究Bcl-xl基因对成人神经母细胞廇SH-SY5Y细胞毒性的影响.【方法】 将构建好的Bcl-xl基因重组真核表达载体pIRES2-EGFP/Bcl-xl转染SH-SY5Y细胞,并分为正常组、pIRES2-EGFP空质粒转染组、pIRES2-EGFP/Bcl-xl质粒转染组,利用凋亡诱导剂硝普钠(50 μmol/L)诱导各组细胞凋亡,行Hoechst33258细胞染色对比观察各组细胞凋亡情况,通过MTT法分析各组细胞存活率?RT-PCR与Western blotting检测转染后Bcl-xl基因的表达情况.【结果】 转染SH-SY5Y细胞后,检测结果显示Bcl-xl基因能够高效转染并在此细胞中蛋白表达，相对于未转染细胞来说,转染细胞能部分对抗硝普钠诱导的凋亡,MTT显示有统计学意义(P < 0.05).【结论】 Bcl-xl基因能够通过升高基因的表达抑制NO诱导的类神经元细胞死亡,同时也能间接说明硝普钠产生的NO对细胞的毒性可能大部分是通过凋亡途径来产生的.
AbstractList 【目的】 研究Bcl-xl基因对成人神经母细胞廇SH-SY5Y细胞毒性的影响.【方法】 将构建好的Bcl-xl基因重组真核表达载体pIRES2-EGFP/Bcl-xl转染SH-SY5Y细胞,并分为正常组、pIRES2-EGFP空质粒转染组、pIRES2-EGFP/Bcl-xl质粒转染组,利用凋亡诱导剂硝普钠(50 μmol/L)诱导各组细胞凋亡,行Hoechst33258细胞染色对比观察各组细胞凋亡情况,通过MTT法分析各组细胞存活率?RT-PCR与Western blotting检测转染后Bcl-xl基因的表达情况.【结果】 转染SH-SY5Y细胞后,检测结果显示Bcl-xl基因能够高效转染并在此细胞中蛋白表达，相对于未转染细胞来说,转染细胞能部分对抗硝普钠诱导的凋亡,MTT显示有统计学意义(P < 0.05).【结论】 Bcl-xl基因能够通过升高基因的表达抑制NO诱导的类神经元细胞死亡,同时也能间接说明硝普钠产生的NO对细胞的毒性可能大部分是通过凋亡途径来产生的.
Author 蒋龙元
刘淑琼
陶恩祥
蔡晓冬
吴秀勤
杨炼红
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  fullname: 吴秀勤
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  fullname: 陶恩祥
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Snippet 【目的】 研究Bcl-xl基因对成人神经母细胞廇SH-SY5Y细胞毒性的影响.【方法】 将构建好的Bcl-xl基因重组真核表达载体pIRES2-EGFP/Bcl-xl转染SH-SY5Y细胞,并分为正常组、pIRES2-EGFP空质粒转染组、pIRES2-EGFP/Bcl-xl质粒转染组,利用凋亡诱导剂硝普钠(50...
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SubjectTerms Bcl-xl基因
SH-SY5Y
凋亡
基因治疗
硝普钠
Title Bcl-xl基因对硝普钠诱导SY5Y细胞凋亡的抑制作用
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Volume 33
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