单肺通气致兔急性肺损伤的蛋白质组学分析

【目的】 探讨兔单肺通气肺损伤后肺组织蛋白组表达变化。【方法】 新西兰白兔12只随机分为双肺通气组(TLV组)和单肺通气组(OLV组),每组6只。TLV组行双肺通气;OLV组行右肺通气,监测氧合指数(OI)。术后对两组左、右肺行组织学检查并评分,对两组左肺组织提取的总蛋白进行双向差分凝胶电泳, DeCyder软件寻找差异表达的蛋白质点,进行质谱分析鉴定。【结果】 OLV组在单肺通气3 h时OI < 300 mmHg,明显低于TLV组(P < 0.05),OLV组左、右肺损伤评分均高于TLV组左、右肺(P < 0.05),OLV组与TLV组之间, 检测并鉴定出6个差异蛋白,包括...

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Published inZhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 33
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Sun Yat-sen University 01.01.2012
Subjects
兔
单肺通气
肺损伤
蛋白组学
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Abstract 【目的】 探讨兔单肺通气肺损伤后肺组织蛋白组表达变化。【方法】 新西兰白兔12只随机分为双肺通气组(TLV组)和单肺通气组(OLV组),每组6只。TLV组行双肺通气;OLV组行右肺通气,监测氧合指数(OI)。术后对两组左、右肺行组织学检查并评分,对两组左肺组织提取的总蛋白进行双向差分凝胶电泳, DeCyder软件寻找差异表达的蛋白质点,进行质谱分析鉴定。【结果】 OLV组在单肺通气3 h时OI < 300 mmHg,明显低于TLV组(P < 0.05),OLV组左、右肺损伤评分均高于TLV组左、右肺(P < 0.05),OLV组与TLV组之间, 检测并鉴定出6个差异蛋白,包括ATP合成酶、肌动蛋白及过氧化还原酶等。【结论】 成功建立兔单肺通气急性肺损伤模型;单肺通气比双肺通气更引起肺损伤、单肺通气肺损伤可引起肺组织蛋白表达改变, 这些差异蛋白主要与能量代谢、细胞骨架及氧化应激相关。
AbstractList 【目的】 探讨兔单肺通气肺损伤后肺组织蛋白组表达变化。【方法】 新西兰白兔12只随机分为双肺通气组(TLV组)和单肺通气组(OLV组),每组6只。TLV组行双肺通气;OLV组行右肺通气,监测氧合指数(OI)。术后对两组左、右肺行组织学检查并评分,对两组左肺组织提取的总蛋白进行双向差分凝胶电泳, DeCyder软件寻找差异表达的蛋白质点,进行质谱分析鉴定。【结果】 OLV组在单肺通气3 h时OI < 300 mmHg,明显低于TLV组(P < 0.05),OLV组左、右肺损伤评分均高于TLV组左、右肺(P < 0.05),OLV组与TLV组之间, 检测并鉴定出6个差异蛋白,包括ATP合成酶、肌动蛋白及过氧化还原酶等。【结论】 成功建立兔单肺通气急性肺损伤模型;单肺通气比双肺通气更引起肺损伤、单肺通气肺损伤可引起肺组织蛋白表达改变, 这些差异蛋白主要与能量代谢、细胞骨架及氧化应激相关。
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SubjectTerms 兔
单肺通气
肺损伤
蛋白组学
Title 单肺通气致兔急性肺损伤的蛋白质组学分析
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