TGF-β Receptor–dependent Tissue Factor Release and Proteomic Profiling of Extracellular Vesicles from Mechanically Compressed Human Bronchial Epithelial Cells

• Published in: American journal of respiratory cell and molecular biology Vol. 73; no. 1; pp. 88 - 95
• Main Authors: Mwase, Chimwemwe, Schworer, Stephen A., Gilmore, Rodney C., Khan, Faria, Dy, Alane Blythe C., Haber, Adam L., Boucher, Richard C., Randell, Scott H., Mackman, Nigel, Park, Jin-Ah
• Format: Journal Article
• Language: English
• Published: United States American Thoracic Society 01.07.2025
• Subjects: Adult; Airway management; Asthma; Asthma - metabolism; Asthma - pathology; Bronchi - metabolism; Bronchi - pathology; Bronchoconstriction; Compression; Epithelial cells; Epithelial Cells - metabolism; Extracellular vesicles; Extracellular Vesicles - metabolism; Female; Humans; Inflammation; Male; Middle Aged; Proteomics; Proteomics - methods; Receptors, Transforming Growth Factor beta - metabolism; Thromboplastin - genetics; Thromboplastin - metabolism; Tissue engineering; Tissue factor; Transforming growth factor-b; asthma exacerbations; TGF-β; epithelial compression; extracellular vesicles; tissue factor

In asthma, tissue factor (TF) concentrations are elevated in the lung. In our previous studies using mechanically compressed human bronchial epithelial (HBE) cells, which are a well-defined model of bronchoconstriction during asthma exacerbations, we detected TF within extracellular vesicles (EVs) r...