Phenolic compounds from in vitro cultures of Rindera gareca Boiss. & Heldr

• Published in: Planta Medica
• Main Authors: Sykłowska-Baranek, K, Pietrosiuk, A, Szyszko, E, Graikou, K, Jeziorek, M, Kuźma, Ł, Chinou, I
• Format: Conference Proceeding
• Language: English
• Published: 19.07.2012
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-0032-1321029

Rindera graeca (Boraginaceae) is an endemic plant of South-East Europe and Mediterranean Basin, growing in rocky places of Greece. The presence of phenolic compounds has been analyzed in established in vitro cultures of shoots, natural and transgenic roots of R.graeca . The content of caffeic acid (CA), rosmarinic acid (RA), lithospermic (LA) and lithospermic B (LAB) acids has been investigated using HPLC-DAD method. RA has a number of interesting biological activities, e.g. antiviral, antibacterial, antiinflammatory and antioxidant. LAB, a tetrametric derivative of caffeic acid, exhibits the endothelium-dependent vasodilator and hypotensive. LA showed antioxidant effects. CA has not been detected in examined plant material while LAB (0.09mg/g DW) has been found only in shoots. The highest content of RA (15mg/g DW) has been also determined in shoots. Among investigated two lines of natural roots (RgKN and RgKN-NOA) and two lines of transgenic roots (RgKT7 and RgKT17) the highest yield of RA and LA have been noted in natural roots derived from growing in vitro seedlings (RgKN), 13mg/g DW and 1mg/g DW, respectively. In transgenic roots the RA and LA content was 9mg/g DW and 0.66mg/g DW, respectively.