Irreversible site‐directed labeling of the 4‐aminobutyrate binding site by tritiated meta‐ sulfonate benzene diazonium Contribution of a nucleophilic amino acid residue of the α1 subunit

• Published in: European journal of biochemistry Vol. 265; no. 1; pp. 189 - 194
• Main Authors: Jacques, Patrice, Perret, Philippe, Bouchet, Marie‐Jeanne, Foucaud, Bernard, Goeldner, Maurice, Benke, Dietmar
• Format: Journal Article
• Language: English
• Published: 01.10.1999

Tritiated meta‐ sulfonate benzene diazonium ([ 3 H]MSBD), a molecule structurally related to 4‐aminobutyrate (GABA), which presents a reactivity toward nucleophilic amino acid residues, was synthesized to investigate the GABA binding site on the GABA A receptor. Irreversible labeling reactions using...