Detection of bacterial endophytes in Vitis vinifera L and antibiotic activity against grapevine fungal pathogens
This chapter presents the results of studies conducted to (i) detect and localize endophytic bacteria in symptomless and symptomatic leaf tissues of grapevine ( ) using fluorescence hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM); and (ii) test the antagonistic act...
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Published in | Biocontrol of Major Grapevine Diseases pp. 182 - 190 |
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Main Authors | , , , |
Format | Book Chapter |
Language | English |
Published |
Wallingford, UK
CABI
2016
CAB International |
Subjects | |
Online Access | Get full text |
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Summary: | This chapter presents the results of studies conducted to (i) detect and localize endophytic bacteria in symptomless and symptomatic leaf tissues of grapevine ( ) using fluorescence hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM); and (ii) test the antagonistic activity of AG1, previously isolated from grape wood affected by the esca syndrome, against grapevine fungal pathogens: , obtained from grapevine leaves showing necrotic concentric spots; and , isolated from grapes affected by secondary rot; , isolated from grapes with grey mould; , agent of grapevine leaf spots; , obtained from grapevines with white rot symptoms; , isolated from the grapevine rhizosphere; , isolated from vine wood with cortical cankers; and , both isolated from grapevine showing esca symptoms; from grape leaves showing necrotic spots; and , obtained from decaying vine. The FISH/CLSM approach was successfully applied to visualize endophytic bacteria in grapevine leaves, including also those that it is not possible to cultivate in sterilized medium. Also, AG1 showed a potential role as a biological control agent against trunk fungal pathogens as well as other fungal phytopathogens. |
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ISBN: | 1780647123 9781780647128 |
DOI: | 10.1079/9781780647128.0182 |