Detection of bacterial endophytes in Vitis vinifera L and antibiotic activity against grapevine fungal pathogens

• Published in: Biocontrol of Major Grapevine Diseases pp. 182 - 190
• Main Authors: Piccolo, S. Lo, Alfonzo, A, Burruano, S, Moschetti, G
• Format: Book Chapter
• Language: English
• Published: Wallingford, UK CABI 2016; CAB International
• Subjects: Agaricomycetes; Agaricomycotina; Alternaria; Alternaria alternata; analytical methods; analytical techniques; angiosperms; Ascomycota; Aspergillus; Aspergillus carbonarius; Aspergillus ochraceus; Bacillaceae; Bacillales; Bacilli; Bacillus (Bacteria); Bacillus amyloliquefaciens; Bacteria; bacterium; Basidiomycota; biocontrol agents; biological control agents; biological control organisms; Botany & plant sciences; Botryosphaeriaceae; Botryosphaeriales; Botrytis; Botrytis cinerea; Capnodiales; Chaetothyriales; detection; Diaporthales; Didymellaceae; Dothideomycetes; endophytes; eudicots; eukaryotes; Eurotiales; Eurotiomycetes; Firmicutes; FISH (cytogenetic techniques); fluorescence in situ hybridisation; fluorescence in situ hybridization; Fomitiporia; Fomitiporia mediterranea; fungal antagonists; fungi; fungus; Fusarium; Fusarium oxysporum; grapes; Helotiales; Herpotrichiellaceae; Hymenochaetaceae; Hymenochaetales; Hypocreales; Lasiodiplodia; Lasiodiplodia theobromae; leaves; Leotiomycetes; microscopy; Mycosphaerellaceae; natural enemies; Nectriaceae; pathogens; Penicillium; Penicillium verrucosum; Pezizomycotina; Phaeoacremonium; Phaeoacremonium aleophilum; Phaeomoniella; Phaeomoniella chlamydospora; Phoma; Phoma glomerata; phytopathogenic fungi; phytopathogens; plant pathogenic fungi; plant pathogens; plants; Plectosphaerellaceae; Pleosporaceae; Pleosporales; prokaryotes; Pseudocercospora; Pseudocercospora vitis; Sclerotiniaceae; Sordariomycetes; Spermatophyta; techniques; Togniniaceae; Trichocomaceae; Verticillium; Verticillium dahliae; Vitaceae; Vitales; Vitis; Vitis vinifera
• ISBN: 1780647123; 9781780647128
• DOI: 10.1079/9781780647128.0182

This chapter presents the results of studies conducted to (i) detect and localize endophytic bacteria in symptomless and symptomatic leaf tissues of grapevine ( ) using fluorescence hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM); and (ii) test the antagonistic activity of AG1, previously isolated from grape wood affected by the esca syndrome, against grapevine fungal pathogens: , obtained from grapevine leaves showing necrotic concentric spots; and , isolated from grapes affected by secondary rot; , isolated from grapes with grey mould; , agent of grapevine leaf spots; , obtained from grapevines with white rot symptoms; , isolated from the grapevine rhizosphere; , isolated from vine wood with cortical cankers; and , both isolated from grapevine showing esca symptoms; from grape leaves showing necrotic spots; and , obtained from decaying vine. The FISH/CLSM approach was successfully applied to visualize endophytic bacteria in grapevine leaves, including also those that it is not possible to cultivate in sterilized medium. Also, AG1 showed a potential role as a biological control agent against trunk fungal pathogens as well as other fungal phytopathogens.