Modulation of the high concentration viscosity of IgG 1 antibodies using clinically validated Fc mutations

• Published in: mAbs Vol. 16; no. 1; p. 2379560
• Main Authors: Heisler, Joel, Kovner, Daniel, Izadi, Saeed, Zarzar, Jonathan, Carter, Paul J
• Format: Journal Article
• Language: English
• Published: United States 31.12.2024
• Subjects: Humans; Immunoglobulin Fab Fragments - chemistry; Immunoglobulin Fab Fragments - genetics; Immunoglobulin Fc Fragments - chemistry; Immunoglobulin Fc Fragments - genetics; Immunoglobulin G - chemistry; Immunoglobulin G - genetics; Mutation; Omalizumab - chemistry; Trastuzumab - chemistry; Viscosity; self-association; viscosity; rheology; subcutaneous delivery; Intermolecular interactions; molecular dynamics; intramolecular interactions

The self-association of therapeutic antibodies can result in elevated viscosity and create problems in manufacturing and formulation, as well as limit delivery by subcutaneous injection. The high concentration viscosity of some antibodies has been reduced by variable domain mutations or by the addit...