Effects of Cyanide on Some Histological and Immunohistochemical Parameters of Common Carp (Cyprinus carpio)
In this study, Bcl 2 and Caspase 3 expressions and histomorphological changes were investigated in the liver, gill and skin tissues of carp (Cyprinus carpio) in which 0,1 mg/L and 0,2 mg/L concentrations of cyanide were added to their environment. It was determined that the lipid accumulation, lymph...
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Published in | Journal of limnology and freshwater fisheries research Vol. 9; no. 2; pp. 115 - 122 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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Egirdir-Isparta
Egirdir Fisheries Research Institute
25.08.2023
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Abstract | In this study, Bcl 2 and Caspase 3 expressions and histomorphological changes were investigated in the liver, gill and skin tissues of carp (Cyprinus carpio) in which 0,1 mg/L and 0,2 mg/L concentrations of cyanide were added to their environment. It was determined that the lipid accumulation, lymphocyte infiltration, fibrosis and regeneration in the liver tissues; hyperplasia, cell aggregates and goblet cells in the skin epithelium and gill filaments of fish exposed to cyanide. As a result of the study, it was observed that Bcl-2 expressions decreased and caspase-3 expressions increased in all tissues of fish exposed to cyanide at concentrations of 0,1 mg/L and 0,2 mg/L. Changes in Bcl-2 and caspase-3 expression levels result in disruption of the apoptosis mechanism in the liver, gill and skin tissues. At the end of the study, it was concluded that the examined parameters were a good indicator for cyanide intoxication.
Bu çalışmada bulundukları ortama 0,1 mg/L ve 0,2 mg/L konsantrasyonda siyanür eklenen sazan balıklarının (Cyprinus carpio) karaciğer, solungaç ve deri dokusu Bcl-2 ve kaspaz-3 ekspresyonları ve histomorfolojik açıdan değişimleri araştırılmıştır. Siyanüre maruz kalan balıkların karaciğer dokularında lipid birikimi, lenfosit infiltrasyonu, fibrosis ve rejenerasyon, deri epitelinde ve solungaç filamentlerinde ise hiperplazi, hücre agregatları ve goblet hücreleri tespit edilmiştir. Yapılan çalışma sonunda 0,1 mg/L ve 0,2 mg/L konsantrasyonlarda siyanüre maruz bırakılan sazan balıklarının incelenen tüm dokularında Bcl-2 ekspresyonlarının azaldığı, kaspaz-3 ekspresyonlarının ise arttığı görülmüştür. Bcl-2 ve kaspaz-3 ekspresyon düzeylerindeki değişimler ilgili dokulardaki apoptosis mekanizmasının bozulması sonucunu doğurmaktadır. Çalışma sonunda incelenen parametrelerin siyanür intoksikasyonu için iyi bir indikatör olduğu sonucuna ulaşılmıştır. |
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AbstractList | In this study, Bcl 2 and Caspase 3 expressions and histomorphological changes were investigated in the liver, gill and skin tissues of carp (Cyprinus carpio) in which 0,1 mg/L and 0,2 mg/L concentrations of cyanide were added to their environment. It was determined that the lipid accumulation, lymphocyte infiltration, fibrosis and regeneration in the liver tissues; hyperplasia, cell aggregates and goblet cells in the skin epithelium and gill filaments of fish exposed to cyanide. As a result of the study, it was observed that Bcl-2 expressions decreased and caspase-3 expressions increased in all tissues of fish exposed to cyanide at concentrations of 0,1 mg/L and 0,2 mg/L. Changes in Bcl-2 and caspase-3 expression levels result in disruption of the apoptosis mechanism in the liver, gill and skin tissues. At the end of the study, it was concluded that the examined parameters were a good indicator for cyanide intoxication.
Bu çalışmada bulundukları ortama 0,1 mg/L ve 0,2 mg/L konsantrasyonda siyanür eklenen sazan balıklarının (Cyprinus carpio) karaciğer, solungaç ve deri dokusu Bcl-2 ve kaspaz-3 ekspresyonları ve histomorfolojik açıdan değişimleri araştırılmıştır. Siyanüre maruz kalan balıkların karaciğer dokularında lipid birikimi, lenfosit infiltrasyonu, fibrosis ve rejenerasyon, deri epitelinde ve solungaç filamentlerinde ise hiperplazi, hücre agregatları ve goblet hücreleri tespit edilmiştir. Yapılan çalışma sonunda 0,1 mg/L ve 0,2 mg/L konsantrasyonlarda siyanüre maruz bırakılan sazan balıklarının incelenen tüm dokularında Bcl-2 ekspresyonlarının azaldığı, kaspaz-3 ekspresyonlarının ise arttığı görülmüştür. Bcl-2 ve kaspaz-3 ekspresyon düzeylerindeki değişimler ilgili dokulardaki apoptosis mekanizmasının bozulması sonucunu doğurmaktadır. Çalışma sonunda incelenen parametrelerin siyanür intoksikasyonu için iyi bir indikatör olduğu sonucuna ulaşılmıştır. In this study, Bcl 2 and Caspase 3 expressions and histomorphological changes were investigated in the liver, gill and skin tissues of carp (Cyprinus carpio) in which 0,1 mg/L and 0,2 mg/L concentrations of cyanide were added to their environment. It was determined that the lipid accumulation, lymphocyte infiltration, fibrosis and regeneration in the liver tissues; hyperplasia, cell aggregates and goblet cells in the skin epithelium and gill filaments of fish exposed to cyanide. As a result of the study, it was observed that Bcl-2 expressions decreased and caspase-3 expressions increased in all tissues of fish exposed to cyanide at concentrations of 0,1 mg/L and 0,2 mg/L. Changes in Bcl2 and caspase-3 expression levels result in disruption of the apoptosis mechanism in the liver, gill and skin tissues. At the end of the study, it was concluded that the examined parameters were a good indicator for cyanide intoxication. |
Author | UYSAL, Kazım DEĞER, Ayşenur KAVASOGLU, Mustafa |
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SubjectTerms | Animal tissues Apoptosis Bcl-2 protein Caspase-3 Cyanides Cyprinus carpio Epithelium Fibrosis Filaments Fish Fish skin Freshwater fishes Gills Goblet cells Hepatocytes Hyperplasia Intoxication Lipids Liver Lymphocytes Parameters Regeneration (biological) Tissue |
Title | Effects of Cyanide on Some Histological and Immunohistochemical Parameters of Common Carp (Cyprinus carpio) |
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