The ligand binding domain of the nicotinic acetylcholine receptor
The interaction of the acetylcholine receptor (AChR) binding site domain with specific antibodies and with α‐bungarotoxin (α‐BTX) has been compared. The cloned and expressed ligand binding domain of the mouse AChR α‐subunit binds α‐BTX, whereas the mongoose‐expressed domain is not recognized by α‐BT...
Saved in:
| Published in | FEBS letters Vol. 318; no. 3; pp. 264 - 268 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
08.03.1993
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | The interaction of the acetylcholine receptor (AChR) binding site domain with specific antibodies and with α‐bungarotoxin (α‐BTX) has been compared. The cloned and expressed ligand binding domain of the mouse AChR α‐subunit binds α‐BTX, whereas the mongoose‐expressed domain is not recognized by α‐BTX. On the other hand, both the mouse and mongoose domains bind to the site‐specific monoclonal antibody 5.5. These results demonstrate that the structural requirements for binding of α‐BTX and mcAb 5.5, both of which interact with the AChR binding site, are distinct from each other. |
|---|---|
| AbstractList | The interaction of the acetylcholine receptor (AChR) binding site domain with specific antibodies and with α‐bungarotoxin (α‐BTX) has been compared. The cloned and expressed ligand binding domain of the mouse AChR α‐subunit binds α‐BTX, whereas the mongoose‐expressed domain is not recognized by α‐BTX. On the other hand, both the mouse and mongoose domains bind to the site‐specific monoclonal antibody 5.5. These results demonstrate that the structural requirements for binding of α‐BTX and mcAb 5.5, both of which interact with the AChR binding site, are distinct from each other. |
| Author | Barchan, Dora Aladjem, Mirit Kachalsky, Sylvia G. Fuchs, Sara |
| Author_xml | – sequence: 1 givenname: Sylvia G. surname: Kachalsky fullname: Kachalsky, Sylvia G. – sequence: 2 givenname: Mirit surname: Aladjem fullname: Aladjem, Mirit – sequence: 3 givenname: Dora surname: Barchan fullname: Barchan, Dora – sequence: 4 givenname: Sara surname: Fuchs fullname: Fuchs, Sara |
| BookMark | eNo9UNFKwzAUDTLBbvoHPuRRH6q5Tdomj3NsKgx8mQ99Ck1ys0W6dHQF6d_bThEu93DPuRw4Z05msY1IyD2wJ2BQPDMGIs1LxR8Uf5Qsz_K0uiIJyJKnXBRyRpL_lxsyP5-_2HhLUAlZ7g5Im7Cvo6MmRBfinrr2WIdIW0_7UYzBtn0YN60t9kNjD20TItIOLZ76trsl175uznj3hwvyuVnvVm_p9uP1fbXcphZAVKkwXjjBPaBXWForLfq64MowafyIgNZIWRqP6Bygkc5mXEjlQXrnJfIF2fz6focGB33qwrHuBg1MTx3oKaCeAupxLh3oSm_WL9kkTLziF7biP_NEV-U |
| CitedBy_id | crossref_primary_10_1016_S0028_3908_99_00116_1 crossref_primary_10_1056_NEJM199406233302507 crossref_primary_10_1016_S0197_0186_99_00154_0 crossref_primary_10_1093_oxfordjournals_molbev_a003967 crossref_primary_10_1016_S0301_4622_00_00114_9 crossref_primary_10_3109_10409239609106586 |
| ContentType | Journal Article |
| Copyright | FEBS Letters 318 (1993) 1873-3468 © 2015 Federation of European Biochemical Societies |
| Copyright_xml | – notice: FEBS Letters 318 (1993) 1873-3468 © 2015 Federation of European Biochemical Societies |
| DOI | 10.1016/0014-5793(93)80525-Y |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Chemistry Biology |
| EISSN | 1873-3468 |
| EndPage | 268 |
| ExternalDocumentID | FEB2001457939380525Y |
| Genre | shortCommunication |
| GroupedDBID | --- --K -~X .55 .~1 0R~ 0SF 1OC 1~5 24P 2WC 4.4 4G. 53G 5GY 5RE 6I. 7-5 71M 8P~ AABNK AACTN AAEDW AAFTH AAHBH AAIKJ AALRI AAXUO ABEFU ABFNM ABFRF ABJNI ABLJU ABMAC ABVKL ACGFO ACGFS ACIUM ACNCT ACXQS ADBBV ADEZE ADKYN ADOZA ADVLN ADZMN AEFWE AEGXH AENEX AFBPY AFFNX AGHFR AI. AIAGR AITUG AKRWK ALMA_UNASSIGNED_HOLDINGS AMRAJ AZFZN BAWUL BMXJE C45 CS3 DIK DRMAN DU5 E3Z EBS EJD EO8 EO9 EP2 EP3 F5P FDB FIRID G-Q GX1 HZ~ IHE IXB J1W L7B LATKE LEEKS LITHE LOXES LUTES LX3 MRFUL MRMAN MSSTM MVM N9A NCXOZ O9- OK1 OVD OZT P2P Q38 RIG ROL RPZ SDF SDG SDP SEL SES SFE TEORI TR2 UHB UNMZH VH1 WBKPD WH7 WIH WIJ WIK WIN X7M Y6R YK3 ZGI ~02 |
| ID | FETCH-LOGICAL-c114Y-4bf4d43f1ef9e7cc8cefa639b08bf6391ecb887bfeedd1eb8dc23489f18fdf8e3 |
| ISSN | 0014-5793 |
| IngestDate | Sat Aug 24 01:04:48 EDT 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c114Y-4bf4d43f1ef9e7cc8cefa639b08bf6391ecb887bfeedd1eb8dc23489f18fdf8e3 |
| PageCount | 5 |
| ParticipantIDs | wiley_primary_10_1016_0014_5793_93_80525_Y_FEB2001457939380525Y |
| PublicationCentury | 1900 |
| PublicationDate | March 08, 1993 |
| PublicationDateYYYYMMDD | 1993-03-08 |
| PublicationDate_xml | – month: 03 year: 1993 text: March 08, 1993 day: 08 |
| PublicationDecade | 1990 |
| PublicationTitle | FEBS letters |
| PublicationYear | 1993 |
| References | 1986; 505 1990; 11 1986; 135 1986; 83 1989; 86 1981; 256 1977; 15 1981; 377 1990; 29 1983; 6 1991; 30 1991; 31 1984; 259 1983; 76 1985; 82 1980 1975; 82 1992; 89 1981; 20 1977; 252 |
| References_xml | – volume: 31 start-page: 37 year: 1991 end-page: 72 publication-title: Annu. Rev. Pharmacol. Toxicol. – volume: 256 start-page: 8294 year: 1981 end-page: 8297 publication-title: J. Biol. Chem. – volume: 29 start-page: 6409 year: 1990 end-page: 6415 publication-title: Biochemistry – volume: 83 start-page: 9250 year: 1986 end-page: 9253 publication-title: Proc. Natl. Acad. Sci. USA – volume: 11 start-page: 485 year: 1990 end-page: 492 publication-title: Trends Pharmacol. Sci. – volume: 82 start-page: 3490 year: 1985 end-page: 3493 publication-title: Proc. Natl. Acad. Sci. USA – volume: 135 start-page: 82 year: 1986 end-page: 89 publication-title: Biochem. Biophys. Res. Commun. – start-page: 191 year: 1980 end-page: 250 – volume: 259 start-page: 11662 year: 1984 end-page: 11665 publication-title: J. Biol. Chem. – volume: 29 start-page: 6730 year: 1990 end-page: 6734 publication-title: Biochemistry – volume: 76 start-page: 123 year: 1983 end-page: 128 publication-title: J. Memb. Biol. – volume: 20 start-page: 5920 year: 1981 end-page: 5924 publication-title: Biochemistry – volume: 252 start-page: 8629 year: 1977 end-page: 8633 publication-title: J. Biol. Chem. – volume: 30 start-page: 4925 year: 1991 end-page: 4934 publication-title: Biochemistry – volume: 6 start-page: 303 year: 1983 end-page: 311 publication-title: Muscle and Nerve – volume: 82 start-page: 3490 year: 1975 end-page: 3493 publication-title: Proc. Natl. Acad. Sci. USA – volume: 377 start-page: 110 year: 1981 end-page: 124 publication-title: Ann. N.Y. Acad. Sci. – volume: 15 start-page: 541 year: 1977 end-page: 548 publication-title: Toxicon – volume: 83 start-page: 3008 year: 1986 end-page: 3011 publication-title: Proc. Natl. Acad. Sci. USA – volume: 505 start-page: 256 year: 1986 end-page: 271 publication-title: Ann. N.Y. Acad. Sci. – volume: 89 start-page: 7717 year: 1992 end-page: 7721 publication-title: Proc. Natl. Acad. Sci. USA – volume: 86 start-page: 7255 year: 1989 end-page: 7259 publication-title: Proc. Natl. Acad. Sci. USA |
| SSID | ssj0001819 |
| Score | 1.458997 |
| Snippet | The interaction of the acetylcholine receptor (AChR) binding site domain with specific antibodies and with α‐bungarotoxin (α‐BTX) has been compared. The cloned... |
| SourceID | wiley |
| SourceType | Publisher |
| StartPage | 264 |
| SubjectTerms | Acetylcholine receptor Ligand binding site Monoclonal antibody 5.5 α-Bungarotoxin |
| Title | The ligand binding domain of the nicotinic acetylcholine receptor |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1016%2F0014-5793%2893%2980525-Y |
| Volume | 318 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb9MwFLZKJwQvaGygcRnyA0KgKqVp4sZ5mtppY2KCB7aJ9SmKbywoS6aSIpVfwU_ecU6crGJIXKQoTa0eW_H5em4-xybk5YSpgCkJTg43Ey9UY-0JpiJPjpTPpGZmpGwc8sPHydFZ-P6cnfd6P29kLS0rMZQ_bq0r-ReuQhvw1VbJ_gVn206hAZ6Bv3AHDsP9j3mcZ19s7FtkWJ6iykvw9d3KfwF8rmzp4yCVulrlVtZZsxLEnL7CfYa7QzoPZieDvK7u6RZ4UnkBb4Hx1ZNV_j1LB--GLUjyVH3VGFPNFlmbQDOrN2BCcVYuWrl_uJQX31wUuos2YG4fXPymBPVDj0V4quFQo9DkUeAFIR6P46Rq0InVzutGGYnbljfqdoxkv0hyDCq0w4G5bW-xPYGBefNOe7kV-5aGrVExpGO_oawVOcyvzSwLLUUc1L-b3yEbY_jK-2Rjevzp83Gr3cEiQpeqGcKVY_qTt23b6zh40wy37vPURsvpJnnQeBt0itB5SHq62CLb0yKtyssVfUXr_N96YWWL3J25p3v77hTAbTIFjFHEGG0wRhFjtDQUMEZbjNE1jFGHsUfk7PDgdP_Ia47d8CQ4x3MvtLmbYWB8bWIdScmlNikYsmLEhYFPX0sBqkkYMK-UrwWHv3oQ8tj43CjDdfCY9Iuy0DuEyiiO9BiaQDOEaQRd2UJtJhiLAFwj84Ts1bOTXOHWKonLObRTmdipTOCqpzKZJ7fx6el_9_CM3O-A_pz0q8VS74KhWYkXDe-vAUFdbm4 |
| link.rule.ids | 315,783,787,27938,27939 |
| linkProvider | Library Specific Holdings |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+ligand+binding+domain+of+the+nicotinic+acetylcholine+receptor&rft.jtitle=FEBS+letters&rft.au=Kachalsky%2C+Sylvia+G.&rft.au=Aladjem%2C+Mirit&rft.au=Barchan%2C+Dora&rft.au=Fuchs%2C+Sara&rft.date=1993-03-08&rft.issn=0014-5793&rft.eissn=1873-3468&rft.volume=318&rft.issue=3&rft.spage=264&rft.epage=268&rft_id=info:doi/10.1016%2F0014-5793%2893%2980525-Y&rft.externalDBID=10.1016%252F0014-5793%252893%252980525-Y&rft.externalDocID=FEB2001457939380525Y |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0014-5793&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0014-5793&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0014-5793&client=summon |