Restoring Memory Function in a Diabetic Mice Model

• Published in: Alzheimer's & dementia Vol. 19; no. S21
• Main Authors: Olayinka, Juliet Nnenda, Akawa, Oluwole Abidemi, OBISESAN, ABIOLA, Awogbindin, Ifeoluwa Oluleke, Green, Grace Igbogi
• Format: Journal Article
• Language: English
• Published: 01.12.2023
• DOI: 10.1002/alz.071636
• ISSN: 1552-5260

Background Diabetes mellitus affects memory function in Alzheimer’s disease (Baglietto‐Vargas et al., 2016). Quercetin (Qt) and vitamin (Vit E) possesses anti‐inflammatory, memory enhancing and anti‐diabetic activities (Bui and Nguyen, 2017). This study explored the effects of administering both quercetin and vitamin E against cognitive deficits and neuroinflamation in high‐fat‐diet (HFD) and streptozotocin (HS)‐induced diabetic mice Method Ninety male mice (n = 15) were divided into six groups and fed with HFD except the normal group for 28 days. Diabetes was induced by STZ (50 mg/kg intraperitoneally) on day 22. Weight and blood glucose levels were taken weekly. On day 15, mice in the pretreatment groups (PQt 12.5 + Vit E 200 mg/kg and PQt 25 + Vit E) commenced treatment while on day 29, mice in the posttreatment groups (PtQt 12.5 + Vit E and PtQt 25 + Vit E) commenced treatment till day 42. Memory function (Y‐maze and NORT) was performed. The hippocampus of the animal brains were harvested for biochemical assays using ELISA (acetylcholinesterase enzyme (AChE), insulin, interleukin‐ 4 (IL‐4), interleukin‐6 (IL‐6) and tumor necrosis factor alpha (TNF‐α). Immunohistochemistry was used in detecting the expression of IBA‐1. Result Quercetin and vitamin E significantly decreased the weight, glucose, insulin, and AChE levels in the HS‐induced mice. Memory function was restored by this combination. IL‐6 was decreased in PQt 25 + V and PtQt 25 +V; TNF‐α was decreased in PQt 25 + V and PtQt 12.5 +V while IL‐4 levels were increased in PQt 25 + V and PtQt 25 +V compared to the HS group. IBA‐1 was scarcely expressed in the PQt 12.5 mg/kg and PtQt (12.5 and 25 mg/kg) group compared to the HSgroup. Conclusion Administering quercetin and vitamin E restored memory function through decreasing the activity of pro‐inflammatory mediators, increasing the activity of anti‐inflammatory mediators and decreasing microglial activation in HS‐induced mice. REFERENCES: Baglietto‐Vargas D, Shi J, Yaeger DM, Ager R, LaFerla FM. Diabetes and Alzheimer’s disease crosstalk. Neuroscience & Biobehavioral Reviews. 2016 May 1;64:272‐87. Bui, T. T., & Nguyen, T. H. (2017). Natural product for the treatment of Alzheimer’s disease. Journal of Basic and Clinical Physiology and Pharmacology, 28(5), 413‐423.