Recombinant adeno-associated virus carrying thymosin β 4 suppresses experimental colitis in mice

• Published in: World journal of gastroenterology : WJG Vol. 23; no. 2; p. 242
• Main Authors: Zheng, Xiao-Yan, Lv, Yi-Fei, Li, Shuang, Li, Qian, Zhang, Qian-Nan, Zhang, Xue-Ting, Hao, Zhi-Ming
• Format: Journal Article
• Language: English
• Published: United States 14.01.2017
• Subjects: Animals; Cell Proliferation; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - metabolism; Colon - enzymology; Colon - metabolism; Crohn Disease - chemically induced; Crohn Disease - metabolism; Dependovirus - genetics; Dextran Sulfate - toxicity; Disease Models, Animal; DNA, Recombinant - administration & dosage; Enterocytes - metabolism; Enterocytes - physiology; Enzyme-Linked Immunosorbent Assay; Genetic Vectors - administration & dosage; Immunochemistry; Interleukin-10 - metabolism; Interleukin-1beta - metabolism; Intestinal Mucosa - cytology; Intestinal Mucosa - enzymology; Intestinal Mucosa - metabolism; Male; Malondialdehyde - metabolism; Mice; Mice, Inbred BALB C; Peroxidase - metabolism; Superoxide Dismutase - metabolism; Thymosin - metabolism; Trinitrobenzenesulfonic Acid - toxicity; Tumor Necrosis Factor-alpha - metabolism; Mice; Colitis; 2,4,6-trinitrobenzene sulfonic acid; Thymosin β4; Dextran sulfate sodium

To investigate the protective effect of a recombinant adeno-associated virus carrying thymosin β (AAV-Tβ ) on murine colitis intracolonic administration. AAV-Tβ was prepared and intracolonically used to mediate the secretory expression of Tβ in mouse colons. Dextran sulfate sodium (DSS) was applied...