Synthetic miRNA Switch Technology Elucidates Heterogeneity in Regulation of Immortalized Megakaryocyte Cell Lines, Associated with Improvement of Platelet Generation Efficiency for Clinical Use

• Published in: Blood Vol. 128; no. 22; p. 3867
• Main Authors: Hashimoto, Kazuya, Matsuura, Satoshi, Fujita, Yoshihiko, Hayashi, Karin, Sugimoto, Naoshi, Yamamoto, Takuya, Saito, Hirohide, Eto, Koji
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 02.12.2016

We have developed a robust in vitro platelet generation system using a self-renewing megakaryocyte (MK) cell line, imMKCL, which was established by introducing three doxycycline (DOX)-inducible transgenes, c-MYC, BMI1, and BCL-XL into human induced pluripotent stem cells (iPSCs)-derived megakaryocyt...