Ultra Rapid, One Step Molecular Detection of BCR-ABL Major and Minor Transcripts by Isothermal RT-Loop Mediated Amplification Reaction

• Published in: Blood Vol. 120; no. 21; p. 2515
• Main Authors: D'agostini, Elena, Minnucci, Giulia, Amicarelli, Giulia, Pultrone, Cinzia, Tettamanzi, Veronica, Boroni, Chiara, Salmoiraghi, Silvia, Spinelli, Orietta, Colotta, Francesco, Rambaldi, Alessandro
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 16.11.2012

Abstract 2515 The molecular identification of the BCRABL transcripts in clinical samples is actually based on a conventional Reverse Transcription Polymerase Chain reaction (RT-PCR). Here we present a novel molecular method, based on Loop Mediated Amplification assay that, starting from RNA (RT-LAMP...