Permeabilization activated reduction in fluorescence: A novel method to measure kinetics of protein interactions with intracellular structures

• Published in: Cytoskeleton (Hoboken, N.J.) Vol. 73; no. 6; p. Spc1
• Main Authors: Singh, Pali P., Hawthorne, Jenci L., Davis, Christie A., Quintero, Omar A.
• Format: Journal Article
• Language: English
• Published: Blackwell Publishing Ltd 01.06.2016
• Subjects: cytoskeleton; digitonin; protein-protein interactions; quantitative fluorescence microscopy; transient kinetic analysis

Understanding kinetic information is fundamental in understanding biological function. Advanced imaging technologies have fostered the development of kinetic analyses in cells. We have developed Permeabilization Activated Reduction in Fluorescence (PARF) analysis for determination of apparent t 1/2...