A ggregatibacter actinomycetemcomitans serotypes, the JP 2 clone and cytolethal distending toxin genes in a T hai population
Abstract Aim To examine the genetic diversity of A ggregatibacter actinomycetemcomitans in T hai adults. Materials and Methods Subgingival plaque samples from 453 subjects were analysed for A . actinomycetemcomitans serotypes, the presence of the high leukotoxin‐producing JP 2 clone and cytolethal d...
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Published in | Journal of clinical periodontology Vol. 39; no. 6; pp. 519 - 525 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.06.2012
|
Online Access | Get full text |
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Summary: | Abstract
Aim
To examine the genetic diversity of
A
ggregatibacter actinomycetemcomitans
in
T
hai adults.
Materials and Methods
Subgingival plaque samples from 453 subjects were analysed for
A
. actinomycetemcomitans
serotypes, the presence of the high leukotoxin‐producing
JP
2 clone and cytolethal distending toxin genes (
cdt
ABC
) using the polymerase chain reaction technique. In subjects who were positive for
cdt
ABC
, restriction fragment length polymorphism analysis was used to identify a single nucleotide polymorphism (
SNP
) in the
cdt
B
gene at amino acid position 281. The extent and severity of periodontal disease were compared between subjects harbouring different
A
. actinomycetemcomitans
genotypes.
Results
Eighty six subjects (19%) were positive for
A
. actinomycetemcomitans
. The
JP
2 clone was not detected. Serotype c was the most prevalent (57%), followed by serotypes a (33%) and b (7%). Among
A
. actinomycetemcomitans
‐positive subjects, 27% were positive for
cdt
ABC
. All
cdt
ABC
‐positive subjects possessed the
SNP
in the
cdt
B
, which is involved with increased toxin activity. The presence of
A
. actinomycetemcomitans
, but not a specific genotype, was significantly related to increased probing depth and periodontal attachment loss.
Conclusions
Our results confirm the previous findings that genotype distribution of
A
. actinomycetemcomitans
varies between ethnic groups. However, no clear relationship between a specific genotype and periodontal conditions was observed. |
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ISSN: | 0303-6979 1600-051X |
DOI: | 10.1111/j.1600-051X.2012.01871.x |