Direct Expression of Fluorinated Proteins in Human Cells for 19 F In-Cell NMR Spectroscopy

• Published in: Journal of the American Chemical Society Vol. 145; no. 2; pp. 1389 - 1399
• Main Authors: Pham, Lan B T, Costantino, Azzurra, Barbieri, Letizia, Calderone, Vito, Luchinat, Enrico, Banci, Lucia
• Format: Journal Article
• Language: English
• Published: United States 18.01.2023
• Subjects: Amino Acids; Fluorine - chemistry; Humans; Magnetic Resonance Spectroscopy - methods; Molecular Chaperones; Nuclear Magnetic Resonance, Biomolecular - methods

In-cell NMR spectroscopy is a powerful approach to study protein structure and function in the native cellular environment. It provides precious insights into the folding, maturation, interactions, and ligand binding of important pharmacological targets directly in human cells. However, its widespre...