Low extracellular Ca 2+ activates a transient Cl − current in chicken ovarian granulosa cells
The effects of low Ca 2+ on ion currents in hen ovarian granulosa cells were examined. A fast activating and inactivating transient outward current (TOC) and a slowly activating outward current (SOC) could be observed. In the presence of normal Ca 2+ concentration (2.5 mM) and with a holding potenti...
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Published in | American Journal of Physiology: Cell Physiology Vol. 279; no. 2; pp. C319 - C325 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
01.08.2000
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Abstract | The effects of low Ca
2+
on ion currents in hen ovarian granulosa cells were examined. A fast activating and inactivating transient outward current (TOC) and a slowly activating outward current (SOC) could be observed. In the presence of normal Ca
2+
concentration (2.5 mM) and with a holding potential of −80 mV, SOC was activated in all cells with command pulses more positive than −20 mV. In 2.5 mM Ca
2+
, TOC appeared in 10% of cells at the command pulse of +80 mV and in 60–85% of cells at +100 to +120 mV. In low-Ca
2+
solution and command potential of +80 mV (holding potential of −80 mV), the amplitude of TOC was enhanced in cells that expressed it in normal Ca
2+
, and TOC appeared in 43% of the cells that did not express it initially in normal Ca
2+
. At both normal and low Ca
2+
levels, TOC decreased as the holding potential became more positive. TOC was reduced in Cl
−
-deficient solution and in the presence of 5-nitro-2-(3-phenylpropylamino)benzoic acid, a Cl
−
channel blocker. These findings suggest that chicken granulosa cells express a Ca
2+
-inactivated TOC carried by Cl
−
. This current may serve as a signal for some of the reduced metabolic functions of granulosa cells associated with Ca
2+
deficiency. |
---|---|
AbstractList | The effects of low Ca
2+
on ion currents in hen ovarian granulosa cells were examined. A fast activating and inactivating transient outward current (TOC) and a slowly activating outward current (SOC) could be observed. In the presence of normal Ca
2+
concentration (2.5 mM) and with a holding potential of −80 mV, SOC was activated in all cells with command pulses more positive than −20 mV. In 2.5 mM Ca
2+
, TOC appeared in 10% of cells at the command pulse of +80 mV and in 60–85% of cells at +100 to +120 mV. In low-Ca
2+
solution and command potential of +80 mV (holding potential of −80 mV), the amplitude of TOC was enhanced in cells that expressed it in normal Ca
2+
, and TOC appeared in 43% of the cells that did not express it initially in normal Ca
2+
. At both normal and low Ca
2+
levels, TOC decreased as the holding potential became more positive. TOC was reduced in Cl
−
-deficient solution and in the presence of 5-nitro-2-(3-phenylpropylamino)benzoic acid, a Cl
−
channel blocker. These findings suggest that chicken granulosa cells express a Ca
2+
-inactivated TOC carried by Cl
−
. This current may serve as a signal for some of the reduced metabolic functions of granulosa cells associated with Ca
2+
deficiency. |
Author | Asem, Elikplimi K. Rane, Stanley G. Qin, Wuxuan |
Author_xml | – sequence: 1 givenname: Wuxuan surname: Qin fullname: Qin, Wuxuan organization: Department of Basic Medical Sciences, School of Veterinary Medicine and – sequence: 2 givenname: Stanley G. surname: Rane fullname: Rane, Stanley G. organization: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1246 – sequence: 3 givenname: Elikplimi K. surname: Asem fullname: Asem, Elikplimi K. organization: Department of Basic Medical Sciences, School of Veterinary Medicine and |
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Cites_doi | 10.1210/endo-129-5-2740 10.1016/0014-5793(88)81053-6 10.1530/jrf.0.0500179 10.1210/endo-125-4-1973 10.1093/jn/125.suppl_7.1965S 10.1016/0016-6480(86)90100-0 10.1095/biolreprod49.1.95 10.1007/BF00724512 10.1210/endo.137.6.8641205 10.1007/BF00374806 10.1016/0303-7207(93)90048-O 10.1002/jcp.1041540113 10.1210/endo-113-2-763 10.1210/endo-114-2-441 10.1016/0092-8674(93)90617-Y 10.1038/366575a0 10.1016/S0300-9629(96)00375-1 10.1095/biolreprod52.3.683 10.1016/0303-7207(86)90019-5 10.1016/S0006-291X(88)80560-6 |
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2+
on ion currents in hen ovarian granulosa cells were examined. A fast activating and inactivating transient outward current (TOC) and a... |
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Title | Low extracellular Ca 2+ activates a transient Cl − current in chicken ovarian granulosa cells |
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