Potencial de rizobactérias na promoção de crescimento e controle da podridãoradicular em alface hidropônica

Os objetivos do trabalho foram avaliar se isolados de rizobactérias do grupo fluorescente do gênero Pseudomonas: 1) produzem metabólitos envolvidos na promoção do crescimento (AIA e HCN); 2) têm potencial para controle biológico de Pythium aphanidermatum; 3) podem promover o crescimento de plantas d...

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Published inSumma phytopathologica Vol. 39; no. 1; pp. 51 - 57
Main Authors Cipriano, Matheus Aparecido Pereira, Patrício, Flávia Rodrigues Alves, Freitas, Sueli dos Santos
Format Journal Article
LanguageEnglish
Published 01.03.2013
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Abstract Os objetivos do trabalho foram avaliar se isolados de rizobactérias do grupo fluorescente do gênero Pseudomonas: 1) produzem metabólitos envolvidos na promoção do crescimento (AIA e HCN); 2) têm potencial para controle biológico de Pythium aphanidermatum; 3) podem promover o crescimento de plantas de alface cultivadas em sistema hidropônico; 4) e verificar se há correspondência nas interações in vitro e in vivo desses microrganismos. Com esses objetivos, placas de Petri contendo meio de cultura ágar-água, com ou sem Pythium aphanidermatum, receberam sementes prégerminadas de alface tratadas com os isolados de rizobactérias. Os comprimentos do hipocótilo e da radícula foram medidos cinco ou sete dias após a incubação a 28 ºC. Dois experimentos foram conduzidos em casa de vegetação com plantas de alface hidropônica e 17 isolados bacterianos. As unidades experimentais receberam a suspensão dos isolados de Pseudomonas spp. e, uma semana depois, a suspensão de zoósporos de P. aphanidermatum. Avaliaram-se o escurecimento das raízes e a massa de matéria seca da parte aérea e das raízes das plantas. Os isolados produzem metabólitos que beneficiam o crescimento de alface mesmo na presença do patógeno. Os isolados LP15, LP25, LP28, LP44 e LP47 reduziram os danos causados por P. aphanidermatum nos experimentos realizados in vitro e in vivo. Este é o primeiro estudo que demonstrou que rizobactérias obtidas de solos brasileiros têm potencial para promover o controle biológico de P. aphanidermatum e o crescimento de plantas de alface em sistemas hidropônicos. The aims of this study were to evaluate whether rhizobacteria with hydroponic lettuce plants and 17 bacterial isolates. The experimental isolates of the fluorescent group of the genus Pseudomonas: 1) units received Pseudomonas spp. isolate suspension and, one week later, produce metabolites involved in growth promotion (AIA and HCN); P. aphanidermatum zoospore suspension. The plants were evaluated for 2) have the potential for the biological control of Pythium root darkening and shoot and root dry matter mass. The isolates produce aphanidermatum; 3) promote the growth of lettuce cultivated in metabolites which benefit the growth of lettuce even in the presence of hydroponic system; 4) show correspondence in the in vitro and in vivo the pathogen. The isolates LP15, LP25, LP28, LP44 and LP47 reduced interactions. Thus, Petri dishes containing agar-water culture medium, the damage caused by P. aphanidermatum in experiments in vitro and in with or without Pythium aphanidermatum, received pre-germinated vivo. This is the first study which demonstrated that rhizobacteria seeds of lettuce treated with the rhizobacteria isolates. The lengths of obtained from Brazilian soils have the potential to promote the the hypocotyl and radicle were measured at five or seven days after biological control of P. aphanidermatum and the growth of lettuce incubation at 28 ºC. Two experiments were carried out in a greenhouse plants in hydroponic systems.
AbstractList Os objetivos do trabalho foram avaliar se isolados de rizobactérias do grupo fluorescente do gênero Pseudomonas: 1) produzem metabólitos envolvidos na promoção do crescimento (AIA e HCN); 2) têm potencial para controle biológico de Pythium aphanidermatum; 3) podem promover o crescimento de plantas de alface cultivadas em sistema hidropônico; 4) e verificar se há correspondência nas interações in vitro e in vivo desses microrganismos. Com esses objetivos, placas de Petri contendo meio de cultura ágar-água, com ou sem Pythium aphanidermatum, receberam sementes prégerminadas de alface tratadas com os isolados de rizobactérias. Os comprimentos do hipocótilo e da radícula foram medidos cinco ou sete dias após a incubação a 28 ºC. Dois experimentos foram conduzidos em casa de vegetação com plantas de alface hidropônica e 17 isolados bacterianos. As unidades experimentais receberam a suspensão dos isolados de Pseudomonas spp. e, uma semana depois, a suspensão de zoósporos de P. aphanidermatum. Avaliaram-se o escurecimento das raízes e a massa de matéria seca da parte aérea e das raízes das plantas. Os isolados produzem metabólitos que beneficiam o crescimento de alface mesmo na presença do patógeno. Os isolados LP15, LP25, LP28, LP44 e LP47 reduziram os danos causados por P. aphanidermatum nos experimentos realizados in vitro e in vivo. Este é o primeiro estudo que demonstrou que rizobactérias obtidas de solos brasileiros têm potencial para promover o controle biológico de P. aphanidermatum e o crescimento de plantas de alface em sistemas hidropônicos. The aims of this study were to evaluate whether rhizobacteria with hydroponic lettuce plants and 17 bacterial isolates. The experimental isolates of the fluorescent group of the genus Pseudomonas: 1) units received Pseudomonas spp. isolate suspension and, one week later, produce metabolites involved in growth promotion (AIA and HCN); P. aphanidermatum zoospore suspension. The plants were evaluated for 2) have the potential for the biological control of Pythium root darkening and shoot and root dry matter mass. The isolates produce aphanidermatum; 3) promote the growth of lettuce cultivated in metabolites which benefit the growth of lettuce even in the presence of hydroponic system; 4) show correspondence in the in vitro and in vivo the pathogen. The isolates LP15, LP25, LP28, LP44 and LP47 reduced interactions. Thus, Petri dishes containing agar-water culture medium, the damage caused by P. aphanidermatum in experiments in vitro and in with or without Pythium aphanidermatum, received pre-germinated vivo. This is the first study which demonstrated that rhizobacteria seeds of lettuce treated with the rhizobacteria isolates. The lengths of obtained from Brazilian soils have the potential to promote the the hypocotyl and radicle were measured at five or seven days after biological control of P. aphanidermatum and the growth of lettuce incubation at 28 ºC. Two experiments were carried out in a greenhouse plants in hydroponic systems.
Author Freitas, Sueli dos Santos
Patrício, Flávia Rodrigues Alves
Cipriano, Matheus Aparecido Pereira
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