De novo phased assembly of the Vitis riparia grape genome
Grapevine is one of the most important fruit species in the world. In order to better understand genetic basis of traits variation and facilitate the breeding of new genotypes, we sequenced, assembled, and annotated the genome of the American native Vitis riparia, one of the main species used worldw...
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17.05.2019
Cold Spring Harbor Laboratory |
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| Abstract | Grapevine is one of the most important fruit species in the world. In order to better understand genetic basis of traits variation and facilitate the breeding of new genotypes, we sequenced, assembled, and annotated the genome of the American native Vitis riparia, one of the main species used worldwide for rootstock and scion breeding. A total of 164 Gb raw DNA reads were obtained from Vitis riparia resulting in a 225X depth of coverage. We generated a genome assembly of the V. riparia grape de novo using the PacBio long-reads that was phased with the 10x Genomics Chromium linked-reads. At the chromosome level, a 500 Mb genome was generated with a scaffold N50 size of 1 Mb. More than 34% of the whole genome were identified as repeat sequences, and 37,207 protein-coding genes were predicted. This genome assembly sets the stage for comparative genomic analysis of the diversification and adaptation of grapevine and will provide a solid resource for further genetic analysis and breeding of this economically important species. Footnotes * https://figshare.com/s/0a52d4408214e9f1e280 * https://www.ncbi.nlm.nih.gov/assembly/GCA_004353265.1 * https://www.ncbi.nlm.nih.gov/bioproject/PRJNA512170 |
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| AbstractList | Grapevine is one of the most important fruit species in the world. In order to better understand genetic basis of traits variation and facilitate the breeding of new genotypes, we sequenced, assembled, and annotated the genome of the American native Vitis riparia, one of the main species used worldwide for rootstock and scion breeding. A total of 164 Gb raw DNA reads were obtained from Vitis riparia resulting in a 225X depth of coverage. We generated a genome assembly of the V. riparia grape de novo using the PacBio long-reads that was phased with the 10x Genomics Chromium linked-reads. At the chromosome level, a 500 Mb genome was generated with a scaffold N50 size of 1 Mb. More than 34% of the whole genome were identified as repeat sequences, and 37,207 protein-coding genes were predicted. This genome assembly sets the stage for comparative genomic analysis of the diversification and adaptation of grapevine and will provide a solid resource for further genetic analysis and breeding of this economically important species. Footnotes * https://figshare.com/s/0a52d4408214e9f1e280 * https://www.ncbi.nlm.nih.gov/assembly/GCA_004353265.1 * https://www.ncbi.nlm.nih.gov/bioproject/PRJNA512170 Grapevine is one of the most important fruit species in the world. In order to better understand genetic basis of traits variation and facilitate the breeding of new genotypes, we sequenced, assembled, and annotated the genome of the American native Vitis riparia, one of the main species used worldwide for rootstock and scion breeding. A total of 164 Gb raw DNA reads were obtained from Vitis riparia resulting in a 225X depth of coverage. We generated a genome assembly of the V. riparia grape de novo using the PacBio long-reads that was phased with the 10x Genomics Chromium linked-reads. At the chromosome level, a 500 Mb genome was generated with a scaffold N50 size of 1 Mb. More than 34% of the whole genome were identified as repeat sequences, and 37,207 protein-coding genes were predicted. This genome assembly sets the stage for comparative genomic analysis of the diversification and adaptation of grapevine and will provide a solid resource for further genetic analysis and breeding of this economically important species. |
| Author | Pierre-Francois, Bert Rubio, Bernadette Girollet, Nabil |
| Author_xml | – sequence: 1 givenname: Nabil surname: Girollet fullname: Girollet, Nabil – sequence: 2 givenname: Bernadette surname: Rubio fullname: Rubio, Bernadette – sequence: 3 givenname: Bert surname: Pierre-Francois fullname: Pierre-Francois, Bert |
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| Copyright | 2019. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2019, Posted by Cold Spring Harbor Laboratory |
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| DOI | 10.1101/640565 |
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| References | Walker (640565v1.41) 2014; 9 Stanke (640565v1.52) 2004; 32 Buels (640565v1.53) 2016; 12 Lodhi, Reisch (640565v1.35) 1995; 90 640565v1.38 640565v1.39 Elshire (640565v1.22) 2011; 6 Marcais, Kingsford (640565v1.37) 2011; 27 Li, Lin, An, Wang, Huang (640565v1.10) 2018; 9 Treangen, Salzberg (640565v1.11) 2012; 13 Korf (640565v1.51) 2004; 5 Li (640565v1.54) 2013; 1 Simão, Waterhouse, Ioannidis, Kriventseva, Zdobnov (640565v1.55) 2015; 31 Weisenfeld, Kumar, Shah, Church, Jaffe (640565v1.42) 2017; 27 Van der Auwera (640565v1.29) 2013; 43 Van Ooijen (640565v1.33) 2006 Ma (640565v1.46) 2018; 129 Jaillon (640565v1.4) 2007; 449 Chaisson, Tesler (640565v1.40) 2012; 13 Velasco (640565v1.5) 2007; 2 Andrews (640565v1.23) 2010 Liu, Shi, Yuan, Hu, Zhang, Li, Li, Chen, Mu, Fan (640565v1.36) 2012 Campbell, Holt, Moore, Yandell (640565v1.49) 2014; 48 Hyma (640565v1.31) 2015; 10 640565v1.27 Peterson, Arick (640565v1.1) 2018 Di Genova (640565v1.8) 2014; 14 Da Silva (640565v1.7) 2013; 25 Grattapaglia, Bertolucci, Sederoff (640565v1.30) 1995; 90 Martin (640565v1.24) 2011; 17 Myles (640565v1.18) 2011; 108 Laucou (640565v1.17) 2018; 13 DePristo (640565v1.28) 2011; 43 Campbell (640565v1.50) 2014; 164 (640565v1.56) 2018 Chin (640565v1.12) 2016; 13 Scheben, Yuan, Edwards (640565v1.3) 2016; 6 Jiao, Schneeberger (640565v1.9) 2017; 36 Roach (640565v1.15) 2018; 14 Marguerit (640565v1.21) 2009; 118 Broman, Wu, Sen, Churchill (640565v1.34) 2003; 19 (640565v1.20) 2019 Vinson (640565v1.14) 2005; 15 Cabanettes, Klopp (640565v1.45) 2018; 6 Li, Durbin (640565v1.26) 2010; 26 Li (640565v1.25) 2009; 25 Tarailo-Graovac, Chen (640565v1.47) 2009; 25 Girollet, Rubio, Bert (640565v1.58) 2019 Roach, Schmidt, Borneman (640565v1.13) 2018; 19 Nguyen, Grondin, Courtois, Gantet (640565v1.2) 2018; 24 Zhou (640565v1.16) 2018 Warren (640565v1.43) 2015; 4 Tang (640565v1.44) 2015; 16 Alkan, Sajjadian, Eichler (640565v1.6) 2010; 8 (640565v1.57) 2019 Stam, Van Ooijen (640565v1.32) 1995 Bao, Kojima, Kohany (640565v1.48) 2015; 6 Migicovsky (640565v1.19) 2016; 17 |
| References_xml | – volume: 15 start-page: 1127 year: 2005 end-page: 35 ident: 640565v1.14 article-title: Assembly of polymorphic genomes: algorithms and application to Ciona savignyi publication-title: Genome Research – volume: 8 start-page: 61 year: 2010 end-page: 65 ident: 640565v1.6 article-title: Limitations of next-generation genome sequence assembly publication-title: Nat. Methods – volume: 17 start-page: 478 year: 2016 ident: 640565v1.19 article-title: Genomic ancestry estimation quantifies use of wild species in grape breeding publication-title: BMC Genomics – volume: 164 start-page: 513 year: 2014 end-page: 24 ident: 640565v1.50 article-title: MAKER-P: a tool kit for the rapid creation, management, and quality control of plant genome annotations publication-title: Plant Physiol – volume: 32 start-page: 309 year: 2004 end-page: 312 ident: 640565v1.52 article-title: AUGUSTUS: a web server for gene finding in eukaryotes publication-title: Nucleic Acids Research – volume: 1 year: 2013 ident: 640565v1.54 article-title: Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM publication-title: arXiv:1303.3997 – volume: 4 start-page: 35 year: 2015 ident: 640565v1.43 article-title: LINKS: Scalable, alignment-free scaffolding of draft genomes with long reads publication-title: Gigascience – year: 2010 ident: 640565v1.23 publication-title: Fastqc: a quality control tool for high throughput sequence data – volume: 19 start-page: 889 year: 2003 end-page: 890 ident: 640565v1.34 article-title: R/qtl: QTL mapping in experimental crosses publication-title: Bioinformatics – volume: 14 start-page: e1007807. year: 2018 ident: 640565v1.15 article-title: Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay publication-title: PLoS Genet – volume: 90 start-page: 11 year: 1995 end-page: 16 ident: 640565v1.35 article-title: Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae publication-title: Theor. Appl. Genet – year: 2019 ident: 640565v1.58 publication-title: De novo phased assembly of the Vitis riparia grape genome – volume: 25 start-page: 4777 year: 2013 end-page: 4788 ident: 640565v1.7 article-title: The high polyphenol content of grapevine cultivar Tannat berries is conferred primarily by genes that are not shared with the reference genome publication-title: Plant Cell – ident: 640565v1.27 – year: 2018 ident: 640565v1.56 – volume: 27 start-page: 764 year: 2011 end-page: 770 ident: 640565v1.37 article-title: A fast, lock-free approach for efficient parallel counting of occurrences of k-mers publication-title: Bioinformatics – volume: 43 start-page: 491 year: 2011 end-page: 498 ident: 640565v1.28 article-title: A framework for variation discovery and genotyping using next-generation DNA sequencing data publication-title: Nat Genet – volume: 48 start-page: 1 year: 2014 end-page: 39 ident: 640565v1.49 article-title: Genome Annotation and Curation Using MAKER and MAKER-P publication-title: Curr Protoc Bioinformatics – volume: 129 start-page: 258 year: 2018 end-page: 267 ident: 640565v1.46 article-title: Phylogenomics, biogeography, and adaptive radiation of grapes publication-title: Molecular phylogenetics and evolution – volume: 6 start-page: 11 year: 2015 ident: 640565v1.48 article-title: Repbase Update, a database of repetitive elements in eukaryotic genomes publication-title: Mobile DNA – volume: 14 start-page: 7 year: 2014 ident: 640565v1.8 article-title: Whole genome comparison between table and wine grapes reveals a comprehensive catalog of structural variants publication-title: BMC Plant Biol – volume: 25 start-page: 2078 year: 2009 end-page: 2079 ident: 640565v1.25 article-title: The Sequence Alignment/Map format and SAMtools publication-title: Bioinformatics – volume: 27 start-page: 757 year: 2017 end-page: 767 ident: 640565v1.42 article-title: Direct determination of diploid genome sequences publication-title: Genome research – year: 2018 ident: 640565v1.1 publication-title: Sequencing Plant Genomes – year: 1995 ident: 640565v1.32 publication-title: JOINMAP version 2.0: software for the calculation of genetic linkage maps – year: 2012 ident: 640565v1.36 article-title: Estimation of genomic characteristics by analyzing k-mer frequency in de novo genome projects publication-title: arXiv preprint arXiv:1308 – volume: 13 start-page: 36 year: 2012 end-page: 46 ident: 640565v1.11 article-title: Repetitive DNA and next-generation sequencing: computational challenges and solutions publication-title: Nat. Rev. Genet – volume: 13 start-page: e0192540 year: 2018 ident: 640565v1.17 article-title: Extended diversity analysis of cultivated grapevine Vitis vinifera with 10K genome-wide SNPs publication-title: PLoS One – volume: 25 start-page: 4 year: 2009 end-page: 10 ident: 640565v1.47 article-title: Using RepeatMasker to identify repetitive elements in genomic sequences publication-title: Curr Protoc Bioinformatics – volume: 19 start-page: 460 year: 2018 ident: 640565v1.13 article-title: Purge Haplotigs: allelic contig reassignment for third-gen diploid genome assemblies publication-title: BMC bioinformatics – volume: 17 start-page: 10 year: 2011 end-page: 12 ident: 640565v1.24 article-title: Cutadapt removes adapter sequences from high-throughput sequencing reads publication-title: EMBnet.journal – volume: 108 start-page: 3530 year: 2011 end-page: 3535 ident: 640565v1.18 article-title: Genetic structure and domestication history of the grape publication-title: Proc Natl Acad Sci U S A – volume: 10 start-page: e0134880 year: 2015 ident: 640565v1.31 article-title: Heterozygous mapping strategy (HetMappS) for high resolution Genotyping-By-Sequencing markers: a case study in grapevine publication-title: PLoS One – volume: 6 start-page: e4958 year: 2018 ident: 640565v1.45 article-title: D-GENIES: dot plot large genomes in an interactive, efficient and simple way publication-title: PeerJ – year: 2019 ident: 640565v1.20 publication-title: The state of the world’s biodiversity for food and agriculture – ident: 640565v1.39 – year: 2019 ident: 640565v1.57 – volume: 118 start-page: 1261 year: 2009 end-page: 1278 ident: 640565v1.21 article-title: Genetic dissection of sex determinism, inflorescence morphology and downy mildew resistance in grapevine publication-title: Theor. Appl. Genet – volume: 43 start-page: 1 year: 2013 end-page: 11 ident: 640565v1.29 article-title: From FastQ Data to High-Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline publication-title: Current Protocols in Bioinformatics – volume: 13 start-page: 238 year: 2012 ident: 640565v1.40 article-title: Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory publication-title: BMC Bioinformatics – volume: 449 start-page: 463 year: 2007 end-page: 467 ident: 640565v1.4 article-title: The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla publication-title: Nature – volume: 13 start-page: 1050 year: 2016 end-page: 1054 ident: 640565v1.12 article-title: Phased diploid genome assembly with single-molecule real-time sequencing publication-title: Nat. Methods – volume: 16 start-page: 3 year: 2015 end-page: 10 ident: 640565v1.44 article-title: ALLMAPS: robust scaffold ordering based on multiple maps publication-title: Genome Biol – volume: 9 start-page: e112963 year: 2014 ident: 640565v1.41 article-title: Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement publication-title: PLoS One – year: 2018 ident: 640565v1.16 article-title: Structural variants, clonal propagation, and genome evolution in grapevine (Vitis vinifera) publication-title: bioRxiv 508119 – volume: 90 start-page: 933 year: 1995 end-page: 947 ident: 640565v1.30 article-title: Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and E. urophylla using a pseudo-testcross mapping strategy and RAPD markers publication-title: Theor. Appl. Genet – year: 2006 ident: 640565v1.33 publication-title: JoinMap® 4.0, Software for the calculation of genetic linkage maps in experimental populations – volume: 6 start-page: e19379 year: 2011 ident: 640565v1.22 article-title: A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species publication-title: PLoS ONE – volume: 5 start-page: 59 year: 2004 ident: 640565v1.51 article-title: Gene finding in novel genomes publication-title: BMC Bioinformatics – volume: 9 start-page: 6 year: 2018 ident: 640565v1.10 article-title: Genome Sequencing and Assembly by Long Reads in Plants publication-title: Genes – ident: 640565v1.38 – volume: 6 start-page: 2 year: 2016 end-page: 10 ident: 640565v1.3 article-title: Advances in genomics for adapting crops to climate change publication-title: Curr. Plant Biol – volume: 31 start-page: 3210 year: 2015 end-page: 3212 ident: 640565v1.55 article-title: BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs publication-title: Bioinformatics – volume: 26 start-page: 589 year: 2010 end-page: 595 ident: 640565v1.26 article-title: Fast and accurate long-read alignment with Burrows–Wheeler transform publication-title: Bioinformatics – volume: 36 start-page: 64 year: 2017 end-page: 70 ident: 640565v1.9 article-title: The impact of third generation genomic technologies on plant genome assembly publication-title: Curr. Opin. Plant Biol – volume: 12 start-page: 17 year: 2016 end-page: 66 ident: 640565v1.53 article-title: JBrowse: a dynamic web platform for genome visualization and analysis publication-title: Genome Biology – volume: 2 start-page: e1326 year: 2007 ident: 640565v1.5 article-title: A High Quality Draft Consensus Sequence of the Genome of a Heterozygous Grapevine Variety publication-title: PLoS ONE – volume: 24 start-page: 263 year: 2018 end-page: 274 ident: 640565v1.2 article-title: Next-generation sequencing accelerates crop gene discovery publication-title: Trends Plant Sci |
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| Title | De novo phased assembly of the Vitis riparia grape genome |
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