Studies in Particle Sorting by Paramecium Cilia Arrays

• Published in: bioRxiv
• Main Authors: Mayne, Richard, Whiting, James, Wheway, Gabrielle, Melhuish, Chris, Adamatzky, Andrew
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 17.02.2017; Cold Spring Harbor Laboratory
• Edition: 1.1
• Subjects: Cell surface; Chemoreception; Cilia; Fluid flow; Mechanotransduction; Microbiology; Microparticles; Organelles; Particulate matter; particle tracking; microparticles; morphological computation; microscopy; parallel manipulation
• ISSN: 2692-8205; 2692-8205
• DOI: 10.1101/109470

Motile cilia are cell-surface organelles whose purposes, in ciliated protists and certain ciliated vertebrate epithelia, include generating fluid flow, chemosensation, mechanosensation and substance uptake. Certain properties of cilia arrays, such as beating synchronisation and manipulation of external proximate particulate matter, are considered emergent, but remain incompletely characterised despite these phenomena having being the subject of extensive modelling. This study constitutes a laboratory experimental characterisation of one of the emergent properties of motile cilia; microparticle manipulation. The work demonstrates through automated videomicrographic particle tracking that interactions between microparticles and somatic cilia arrays of the ciliated model organism Paramecium caudatum constitute a form of rudimentary sorting . Small particles are drawn into the organism's proximity by cilia-induced fluid currents at all times, whereas larger particles may be held immobile at a distance from the cell margin when the cell generates characteristic feeding currents in the surrounding media. These findings can contribute to the design and fabrication of biomimetic cilia, with potential applications to the study of ciliopathies.