Macromolecular Crowding-Affected Mobility of Bcnt/Cfdp1, a Disordered Component of the Srcap Complex, from the Nucleus to Cytosol in Subcellular Fractionation

• Published in: bioRxiv
• Main Authors: Iwashita, Shintaro, Suzuki, Takehiro, Nishimura, Kouichi, Onishi, Ayaka, Tsukuda, Kyoka, Kakihara, Yoshito, Kiriyama, Yoshimitsu, Ohoka, Yoshiharu, Nakashima, Kentaro
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Laboratory 08.05.2025
• Edition: 1.2
• Subjects: Biochemistry; Nucleus-cytosol mobility; Western blot; Intracellular behavior; Wnt signaling; Quantitative proteomic analysis; digitonin

Metazoan Bucentaur/Craniofacial Development Protein 1 (Bcnt/Cfdp1) is crucial for embryonic development, and its yeast homolog protein, SWC5, functions as part of the SWR1 complex in histone exchange reactions. Bcnt/Cfdp1, an intrinsically disordered protein (IDP) subject to various post-translation...