Construction of a cellulase hyper-expression system in Trichoderma reesei by promoter and enzyme engineering

• Published in: Microbial cell factories Vol. 11; no. 1; p. 21
• Main Authors: Zou, Gen, Shi, Shaohua, Jiang, Yanping, van den Brink, Joost, de Vries, Ronald P, Chen, Ling, Zhang, Jun, Ma, Liang, Wang, Chengshu, Zhou, Zhihua
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.02.2012; BioMed Central; BMC
• Subjects: Bacteria; Bacterial Proteins - genetics; Binding Sites; Biodegradation; Carbon; Catabolites; Cell culture; Cellulase; Cellulase - genetics; Cellulase - metabolism; Construction; DNA binding proteins; Efficiency; Endoglucanase; Endoplasmic reticulum; Enzymatic activity; Enzyme engineering; Enzymes; Fusion protein; Gene expression; Glucose; Green fluorescent protein; Heterologous expression; Hybrids; Hydrolysates; Hydrolysis; Hypocrea jecorina; mRNA stability; Mutation; Promoter Regions, Genetic; Promoters; Protein Engineering; Proteins; Proteolysis; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Repressors; Site-specific mutagenesis; Stover; Sugar; Thermal stability; Trans-Activators - genetics; Transcription factors; Transcriptional regulation; Trichoderma - enzymology

Trichoderma reesei is the preferred organism for producing industrial cellulases. However, a more efficient heterologous expression system for enzymes from different organism is needed to further improve its cellulase mixture. The strong cbh1 promoter of T. reesei is frequently used in heterologous...