Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations

• Published in: BMC biotechnology Vol. 8; no. 1; p. 2
• Main Authors: Wegerer, Angelika, Sun, Tianqi, Altenbuchner, Josef
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 14.01.2008; BioMed Central; BMC
• Subjects: Biotechnology - methods; Design and construction; Escherichia coli; Escherichia coli - genetics; Gene expression; Genetic aspects; Genetic vectors; Genetic Vectors - genetics; Green Fluorescent Proteins - metabolism; Plasmids - genetics; Promoter Regions, Genetic - genetics; Rhamnose - genetics; Rhamnose - metabolism; Germany

A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. We examined miscellaneous variations of a rhaPBAD expression vector. The introduction of...