Characterization of murine macrophages from bone marrow, spleen and peritoneum

• Published in: BMC immunology Vol. 14; no. 1; p. 6
• Main Authors: Wang, Changqi, Yu, Xiao, Cao, Qi, Wang, Ya, Zheng, Guoping, Tan, Thian Kui, Zhao, Hong, Zhao, Ye, Wang, Yiping, Harris, David CH
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 05.02.2013; BioMed Central; BMC
• Subjects: Animals; Antigen Presentation - immunology; Bone marrow; Bone Marrow Cells - cytology; Bone morphogenetic proteins; CD4-Positive T-Lymphocytes - immunology; Cell Membrane - metabolism; Cell Proliferation; Cell Shape; Cells, Cultured; Comparative analysis; Cytokines - genetics; Cytokines - metabolism; Dextrans - metabolism; Flow Cytometry; Fluorescein-5-isothiocyanate - analogs & derivatives; Fluorescein-5-isothiocyanate - metabolism; Gene Expression Regulation; Genotype & phenotype; Immune response; Lymphocyte Activation - immunology; Macrophage; Macrophages; Macrophages - cytology; Macrophages - immunology; Macrophages, Peritoneal - cytology; Male; Mice; Mice, Inbred BALB C; Nitric oxide; Peritoneum; Phagocytosis - immunology; Phenotype; Physiological aspects; Proteins; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Spleen; Spleen - cytology; Statistical methods; Studies; Transforming growth factors; Australia

Macrophages have heterogeneous phenotypes and complex functions within both innate and adaptive immune responses. To date, most experimental studies have been performed on macrophages derived from bone marrow, spleen and peritoneum. However, differences among macrophages from these particular source...