Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae

• Published in: Microbial cell factories Vol. 10; no. 1; p. 2
• Main Authors: Hasunuma, Tomohisa, Sanda, Tomoya, Yamada, Ryosuke, Yoshimura, Kazuya, Ishii, Jun, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.01.2011; BioMed Central; BMC
• Subjects: Acetic acid; Acetic Acid - pharmacology; Acids; Biofuels; Brewer's yeast; Drug tolerance; Enzymes; Ethanol; Ethanol - metabolism; Fermentation; Formates - pharmacology; Formic acid; Genetic aspects; Genetic Engineering; Hydrolysates; Hydrolysis; lignocellulose; metabolic engineering; Metabolic Networks and Pathways; Metabolic pathways; Metabolites; Metabolome; Metabolomics; Microbiology; Organic acids; Pentose Phosphate Pathway; Physiological aspects; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Solubilization; Stress; Toxicity; Transaldolase; Transaldolase - genetics; Transaldolase - metabolism; Transketolase; Transketolase - genetics; Transketolase - metabolism; Xylose; Xylose - metabolism; Yeasts; Japan

The development of novel yeast strains with increased tolerance toward inhibitors in lignocellulosic hydrolysates is highly desirable for the production of bio-ethanol. Weak organic acids such as acetic and formic acids are necessarily released during the pretreatment (i.e. solubilization and hydrol...