Sources of variability and effect of experimental approach on expression profiling data interpretation

• Published in: BMC bioinformatics Vol. 3; no. 1; p. 4
• Main Authors: Bakay, Marina, Chen, Yi-Wen, Borup, Rehannah, Zhao, Po, Nagaraju, Kanneboyina, Hoffman, Eric P
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 31.01.2002; BioMed Central; BMC
• Subjects: Analysis; Animals; Care and treatment; Cell Line; Child; Child, Preschool; Computational Biology - methods; Control; Data Interpretation, Statistical; Diagnosis; DNA microarrays; Duchenne muscular dystrophy; Dystrophin - deficiency; Dystrophin - genetics; Female; Gene expression; Gene Expression Profiling - methods; Gene Expression Profiling - standards; Gene Expression Profiling - statistics & numerical data; Genetic aspects; Genetic Variation; Humans; Identification and classification; Male; Mice; Mice, Mutant Strains; Muscles - chemistry; Muscles - pathology; Muscular Dystrophy, Duchenne - genetics; Muscular Dystrophy, Duchenne - pathology; Oligonucleotide Array Sequence Analysis - methods; Oligonucleotide Array Sequence Analysis - standards; Oligonucleotide Array Sequence Analysis - statistics & numerical data; Polymorphism, Single Nucleotide - genetics; Research article; RNA - genetics; Sensitivity and Specificity; Spleen - chemistry; Spleen - pathology; Yeast fungi; United States

We provide a systematic study of the sources of variability in expression profiling data using 56 RNAs isolated from human muscle biopsies (34 Affymetrix MuscleChip arrays), and 36 murine cell culture and tissue RNAs (42 Affymetrix U74Av2 arrays). We studied muscle biopsies from 28 human subjects as...