Refining transcriptional programs in kidney development by integration of deep RNA-sequencing and array-based spatial profiling

• Published in: BMC genomics Vol. 12; no. 1; p. 441
• Main Authors: Thiagarajan, Rathi D, Cloonan, Nicole, Gardiner, Brooke B, Mercer, Tim R, Kolle, Gabriel, Nourbakhsh, Ehsan, Wani, Shivangi, Tang, Dave, Krishnan, Keerthana, Georgas, Kylie M, Rumballe, Bree A, Chiu, Han S, Steen, Jason A, Mattick, John S, Little, Melissa H, Grimmond, Sean M
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 05.09.2011; BioMed Central; BMC
• Subjects: Alternative Splicing; Animals; Binding sites; Colleges & universities; Embryonic development; Exons; Gene expression; Genomes; Genomics; High-Throughput Nucleotide Sequencing - methods; Hybridization; Kidney - embryology; Kidney - metabolism; kidney development; Kidneys; mesenchymal-epithelial transition; Mice; microarray; MicroRNAs - genetics; miR-214, microRNA; Organogenesis; Physiological aspects; Polymerase chain reaction; RNA; RNA, Antisense - genetics; RNA, Messenger - genetics; RNA-Seq; sense-antisense transcripts; Sequence Analysis, RNA - methods; Six2, Wt1; Transcription, Genetic; Transcriptome; Australia

The developing mouse kidney is currently the best-characterized model of organogenesis at a transcriptional level. Detailed spatial maps have been generated for gene expression profiling combined with systematic in situ screening. These studies, however, fall short of capturing the transcriptional c...