Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori. Proteomics studies reveal changes in the secretory pathway

• Published in: Microbial cell factories Vol. 11; no. 1; p. 5
• Main Authors: Kosalková, Katarina, García-Estrada, Carlos, Barreiro, Carlos, Flórez, Martha G, Jami, Mohammad S, Paniagua, Miguel A, Martín, Juan F
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.01.2012; BioMed Central; BMC
• Subjects: Acids; Amino acids; Animals; Aspergillus; Aspergillus - enzymology; Aspergillus - genetics; Aspergillus - metabolism; Aspergillus awamori; Casein; casein phosphopeptides; Caseins - pharmacology; Cattle; Chymosin; Chymosin - biosynthesis; Chymosin - genetics; Chymosin - metabolism; Construction; Cytoskeleton; Enzymes; extracellular proteins; filamentous fungi; Food sources; Fungal Proteins - biosynthesis; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene expression; Genetic aspects; Glyceraldehyde-3-phosphate dehydrogenase; Mascots; Oxidative metabolism; Peptides; Phosphopeptides - pharmacology; Phosphorylation; phosphoserine; Physiological aspects; Protein Precursors - genetics; Protein Precursors - metabolism; Proteins; Proteomics; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; secretome; Secretory Pathway - drug effects; secretory pathways; Supplementation; Traffic congestion; Transcription; Vesicles; Spain

The secretion of heterologous animal proteins in filamentous fungi is usually limited by bottlenecks in the vesicle-mediated secretory pathway. Using the secretion of bovine chymosin in Aspergillus awamori as a model, we found a drastic increase (40 to 80-fold) in cells grown with casein or casein p...