versatile toolkit for high throughput functional genomics with Trichoderma reesei

• Published in: Biotechnology for biofuels Vol. 5; no. 1; p. 1
• Main Authors: Schuster, André, Bruno, Kenneth S, Collett, James R, Baker, Scott E, Seiboth, Bernhard, Kubicek, Christian P, Schmoll, Monika
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 02.01.2012; BioMed Central; BioMed Central Ltd; BMC
• Subjects: Ascomycetes; Bacteria; Biofuels; Biotechnology; Cellulase; crossing; DNA repair; Efficiency; electroporation; endo-1,4-beta-glucanase; gene deletion; gene knock-out library; genes; Genetic engineering; Genomes; genomics; Hypocrea jecorina; Libraries; mutagenesis; mutants; Mutation; screening; sexual crossing; sexual development; spores; Technological change; transformation; Trichoderma reesei; Trichoderma reesei; Hypocrea jecorina; vector construction; Yeast; Yeasts

BACKGROUND: The ascomycete fungus, Trichoderma reesei (anamorph of Hypocrea jecorina), represents a biotechnological workhorse and is currently one of the most proficient cellulase producers. While strain improvement was traditionally accomplished by random mutagenesis, a detailed understanding of c...