Assessment of SARS-CoV-2 infectivity of upper respiratory specimens from COVID-19 patients by virus isolation using VeroE6/TMPRSS2 cells

• Published in: BMJ open respiratory research Vol. 8; no. 1; p. e000830
• Main Authors: Yamada, Souichi, Fukushi, Shuetsu, Kinoshita, Hitomi, Ohnishi, Makoto, Suzuki, Tadaki, Fujimoto, Tsuguto, Saijo, Masayuki, Maeda, Ken, Hanaoka, Nozomu, Nojiri, Naomi, Kawana-Tachikawa, Ai, Kusagawa, Shigeru, Ishikawa, Koichi, Harada, Shigeyoshi, Matsuoka, Saori, Kikuchi, Tadashi, Ishii, Hiroshi, Seki, Sayuri, Nakamura-Hoshi, Midori, Miki, Shoji, Runtuwene, Lucky Ronald, Koizumi, Nobuo, Iyoda, Sunao, Takahashi, Hideyuki, Izumiya, Hidemasa, Mitobe, Jiro, Yamamoto, Shouji, Morita, Masatomo, Lee, Ken-ichi, Shimuta, Ken, Saito, Kyoko, Fukasawa, Masayoshi, Hoshino, Yasutaka, Miyazawa, Ken, Nagi, Minoru, Shimokawa, Chikako, Morishima, Yasuyuki, Sakudoh, Takashi, Kaku, Yoshihiro, Lim, Chang Kweng, Tajima, Shigeru, Maeki, Takahiro, Nakayama, Eri, Taniguchi, Satoshi, Ogawa, Motohiko, Kato, Takanobu, Aly, Hussein Hassan, Wakae, Kousho, Fukano, Kento
• Format: Journal Article
• Language: English
• Published: England British Thoracic Society 01.02.2021; BMJ Publishing Group LTD; BMJ Publishing Group
• Subjects: Animals; Cell culture; Cell Line; Chlorocebus aethiops; Coronaviruses; COVID-19; COVID-19 - transmission; COVID-19 Nucleic Acid Testing; Cytopathogenic Effect, Viral; Disease transmission; Humans; Laboratories; Nasal Cavity - virology; Nasopharynx - virology; Public health; Respiratory Infection; Saliva - virology; SARS-CoV-2 - isolation & purification; SARS-CoV-2 - pathogenicity; Serine Endopeptidases - genetics; Severe acute respiratory syndrome coronavirus 2; Specimen Handling; Tomography; Vero Cells; viral infection; Viral infections; Viruses; Japan; COVID-19; viral infection

BackgroundAn outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmissi...