Assessment of SARS-CoV-2 infectivity of upper respiratory specimens from COVID-19 patients by virus isolation using VeroE6/TMPRSS2 cells

BackgroundAn outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmissi...

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Published inBMJ open respiratory research Vol. 8; no. 1; p. e000830
Main Authors Yamada, Souichi, Fukushi, Shuetsu, Kinoshita, Hitomi, Ohnishi, Makoto, Suzuki, Tadaki, Fujimoto, Tsuguto, Saijo, Masayuki, Maeda, Ken, Hanaoka, Nozomu, Nojiri, Naomi, Kawana-Tachikawa, Ai, Kusagawa, Shigeru, Ishikawa, Koichi, Harada, Shigeyoshi, Matsuoka, Saori, Kikuchi, Tadashi, Ishii, Hiroshi, Seki, Sayuri, Nakamura-Hoshi, Midori, Miki, Shoji, Runtuwene, Lucky Ronald, Koizumi, Nobuo, Iyoda, Sunao, Takahashi, Hideyuki, Izumiya, Hidemasa, Mitobe, Jiro, Yamamoto, Shouji, Morita, Masatomo, Lee, Ken-ichi, Shimuta, Ken, Saito, Kyoko, Fukasawa, Masayoshi, Hoshino, Yasutaka, Miyazawa, Ken, Nagi, Minoru, Shimokawa, Chikako, Morishima, Yasuyuki, Sakudoh, Takashi, Kaku, Yoshihiro, Lim, Chang Kweng, Tajima, Shigeru, Maeki, Takahiro, Nakayama, Eri, Taniguchi, Satoshi, Ogawa, Motohiko, Kato, Takanobu, Aly, Hussein Hassan, Wakae, Kousho, Fukano, Kento
Format Journal Article
LanguageEnglish
Published England British Thoracic Society 01.02.2021
BMJ Publishing Group LTD
BMJ Publishing Group
Subjects
Animals
Cell culture
Cell Line
Chlorocebus aethiops
Coronaviruses
COVID-19
COVID-19 - transmission
COVID-19 Nucleic Acid Testing
Cytopathogenic Effect, Viral
Disease transmission
Humans
Laboratories
Nasal Cavity - virology
Nasopharynx - virology
Public health
Respiratory Infection
Saliva - virology
SARS-CoV-2 - isolation & purification
SARS-CoV-2 - pathogenicity
Serine Endopeptidases - genetics
Severe acute respiratory syndrome coronavirus 2
Specimen Handling
Tomography
Vero Cells
viral infection
Viral infections
Viruses
Japan
COVID-19
viral infection
Online AccessGet full text

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Abstract BackgroundAn outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmission from patients with COVID-19; accordingly, viral isolation assays in cell culture are an important aspect of laboratory diagnostics for COVID-19.MethodsWe developed a simple and rapid protocol for isolating SARS-CoV-2 from respiratory specimens using VeroE6/TMPRSS2 cells, a cell line that is highly susceptible to the virus. We also investigated a correlation between isolation of SARS-CoV-2 and viral load detected by real-time RT-PCR (rRT-PCR) using N2 primer/probe set that has been developed for testing of COVID-19 in Japan.ResultsThe SARS-CoV-2 isolation protocol did not require blind passage of inoculated cells and yielded the results of viral isolation within 7 days after inoculation. Specimens with cycle threshold (Ct) values of <20.2, determined by rRT-PCR, were predicted to be isolation-positive. On the other hand, 6.9% of specimens with Ct values >35 were virus isolation-positive, indicating that low viral loads (high Ct values) in upper respiratory specimens do not always indicate no risk of containing transmissible virus.ConclusionIn combination with rRT-PCR, the SARS-CoV-2 isolation protocol provides a means for assessing the potential risk of transmissible virus in upper respiratory specimens.
AbstractList Background An outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmission from patients with COVID-19; accordingly, viral isolation assays in cell culture are an important aspect of laboratory diagnostics for COVID-19.Methods We developed a simple and rapid protocol for isolating SARS-CoV-2 from respiratory specimens using VeroE6/TMPRSS2 cells, a cell line that is highly susceptible to the virus. We also investigated a correlation between isolation of SARS-CoV-2 and viral load detected by real-time RT-PCR (rRT-PCR) using N2 primer/probe set that has been developed for testing of COVID-19 in Japan.Results The SARS-CoV-2 isolation protocol did not require blind passage of inoculated cells and yielded the results of viral isolation within 7 days after inoculation. Specimens with cycle threshold (Ct) values of <20.2, determined by rRT-PCR, were predicted to be isolation-positive. On the other hand, 6.9% of specimens with Ct values >35 were virus isolation-positive, indicating that low viral loads (high Ct values) in upper respiratory specimens do not always indicate no risk of containing transmissible virus.Conclusion In combination with rRT-PCR, the SARS-CoV-2 isolation protocol provides a means for assessing the potential risk of transmissible virus in upper respiratory specimens.
BackgroundAn outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmission from patients with COVID-19; accordingly, viral isolation assays in cell culture are an important aspect of laboratory diagnostics for COVID-19.MethodsWe developed a simple and rapid protocol for isolating SARS-CoV-2 from respiratory specimens using VeroE6/TMPRSS2 cells, a cell line that is highly susceptible to the virus. We also investigated a correlation between isolation of SARS-CoV-2 and viral load detected by real-time RT-PCR (rRT-PCR) using N2 primer/probe set that has been developed for testing of COVID-19 in Japan.ResultsThe SARS-CoV-2 isolation protocol did not require blind passage of inoculated cells and yielded the results of viral isolation within 7 days after inoculation. Specimens with cycle threshold (Ct) values of <20.2, determined by rRT-PCR, were predicted to be isolation-positive. On the other hand, 6.9% of specimens with Ct values >35 were virus isolation-positive, indicating that low viral loads (high Ct values) in upper respiratory specimens do not always indicate no risk of containing transmissible virus.ConclusionIn combination with rRT-PCR, the SARS-CoV-2 isolation protocol provides a means for assessing the potential risk of transmissible virus in upper respiratory specimens.
An outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmission from patients with COVID-19; accordingly, viral isolation assays in cell culture are an important aspect of laboratory diagnostics for COVID-19.BACKGROUNDAn outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmission from patients with COVID-19; accordingly, viral isolation assays in cell culture are an important aspect of laboratory diagnostics for COVID-19.We developed a simple and rapid protocol for isolating SARS-CoV-2 from respiratory specimens using VeroE6/TMPRSS2 cells, a cell line that is highly susceptible to the virus. We also investigated a correlation between isolation of SARS-CoV-2 and viral load detected by real-time RT-PCR (rRT-PCR) using N2 primer/probe set that has been developed for testing of COVID-19 in Japan.METHODSWe developed a simple and rapid protocol for isolating SARS-CoV-2 from respiratory specimens using VeroE6/TMPRSS2 cells, a cell line that is highly susceptible to the virus. We also investigated a correlation between isolation of SARS-CoV-2 and viral load detected by real-time RT-PCR (rRT-PCR) using N2 primer/probe set that has been developed for testing of COVID-19 in Japan.The SARS-CoV-2 isolation protocol did not require blind passage of inoculated cells and yielded the results of viral isolation within 7 days after inoculation. Specimens with cycle threshold (Ct) values of <20.2, determined by rRT-PCR, were predicted to be isolation-positive. On the other hand, 6.9% of specimens with Ct values >35 were virus isolation-positive, indicating that low viral loads (high Ct values) in upper respiratory specimens do not always indicate no risk of containing transmissible virus.RESULTSThe SARS-CoV-2 isolation protocol did not require blind passage of inoculated cells and yielded the results of viral isolation within 7 days after inoculation. Specimens with cycle threshold (Ct) values of <20.2, determined by rRT-PCR, were predicted to be isolation-positive. On the other hand, 6.9% of specimens with Ct values >35 were virus isolation-positive, indicating that low viral loads (high Ct values) in upper respiratory specimens do not always indicate no risk of containing transmissible virus.In combination with rRT-PCR, the SARS-CoV-2 isolation protocol provides a means for assessing the potential risk of transmissible virus in upper respiratory specimens.CONCLUSIONIn combination with rRT-PCR, the SARS-CoV-2 isolation protocol provides a means for assessing the potential risk of transmissible virus in upper respiratory specimens.
An outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmission from patients with COVID-19; accordingly, viral isolation assays in cell culture are an important aspect of laboratory diagnostics for COVID-19. We developed a simple and rapid protocol for isolating SARS-CoV-2 from respiratory specimens using VeroE6/TMPRSS2 cells, a cell line that is highly susceptible to the virus. We also investigated a correlation between isolation of SARS-CoV-2 and viral load detected by real-time RT-PCR (rRT-PCR) using N2 primer/probe set that has been developed for testing of COVID-19 in Japan. The SARS-CoV-2 isolation protocol did not require blind passage of inoculated cells and yielded the results of viral isolation within 7 days after inoculation. Specimens with cycle threshold (Ct) values of <20.2, determined by rRT-PCR, were predicted to be isolation-positive. On the other hand, 6.9% of specimens with Ct values >35 were virus isolation-positive, indicating that low viral loads (high Ct values) in upper respiratory specimens do not always indicate no risk of containing transmissible virus. In combination with rRT-PCR, the SARS-CoV-2 isolation protocol provides a means for assessing the potential risk of transmissible virus in upper respiratory specimens.
Author Fukano, Kento
Nakamura-Hoshi, Midori
Fujimoto, Tsuguto
Sakudoh, Takashi
Saito, Kyoko
Harada, Shigeyoshi
Iyoda, Sunao
Nojiri, Naomi
Kawana-Tachikawa, Ai
Maeki, Takahiro
Saijo, Masayuki
Ishii, Hiroshi
Nagi, Minoru
Yamada, Souichi
Lim, Chang Kweng
Kato, Takanobu
Matsuoka, Saori
Aly, Hussein Hassan
Hoshino, Yasutaka
Runtuwene, Lucky Ronald
Shimuta, Ken
Mitobe, Jiro
Nakayama, Eri
Shimokawa, Chikako
Ishikawa, Koichi
Ohnishi, Makoto
Miyazawa, Ken
Ogawa, Motohiko
Miki, Shoji
Seki, Sayuri
Kinoshita, Hitomi
Wakae, Kousho
Kikuchi, Tadashi
Koizumi, Nobuo
Fukushi, Shuetsu
Maeda, Ken
Yamamoto, Shouji
Morita, Masatomo
Morishima, Yasuyuki
Taniguchi, Satoshi
Suzuki, Tadaki
Kaku, Yoshihiro
Lee, Ken-ichi
Fukasawa, Masayoshi
Kusagawa, Shigeru
Tajima, Shigeru
Takahashi, Hideyuki
Hanaoka, Nozomu
Izumiya, Hidemasa
AuthorAffiliation 5 Department of Veterinary Science , NIID , Shinjuku-ku , Tokyo , Japan
4 Center for Infectious Disease Risk Management , NIID , Shinjuku-ku , Tokyo , Japan
3 Department of Pathology , NIID , Shinjuku-ku , Tokyo , Japan
2 NIID , Shinjuku-ku , Tokyo , Japan
1 Virology 1 , NIID , Shinjuku-ku , Tokyo , Japan
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/33627333$$D View this record in MEDLINE/PubMed
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Cites_doi 10.1093/cid/ciaa638
10.1056/NEJMoa2001316
10.1038/s41564-020-0695-z
10.1007/s10096-020-03913-9
10.1016/j.jcv.2020.104499
10.7883/yoken.JJID.2020.061
10.1073/pnas.2002589117
10.1038/s41586-020-2196-x
10.1128/JCM.00557-20
10.1016/j.jcv.2020.104474
10.1038/s41586-020-2008-3
10.2807/1560-7917.ES.2020.25.32.2001483
10.1007/sl0096-020-03913-9
ContentType Journal Article
Contributor Ishikawa, Koichi
Miyazawa, Ken
Fukano, Kento
Ogawa, Motohiko
Nakamura-Hoshi, Midori
Miki, Shoji
Seki, Sayuri
Wakae, Kousho
Sakudoh, Takashi
Kikuchi, Tadashi
Saito, Kyoko
Harada, Shigeyoshi
Iyoda, Sunao
Nojiri, Naomi
Koizumi, Nobuo
Kawana-Tachikawa, Ai
Maeki, Takahiro
Lee, Ken-Ichi
Yamamoto, Shouji
Morita, Masatomo
Morishima, Yasuyuki
Taniguchi, Satoshi
Kaku, Yoshihiro
Fukasawa, Masayoshi
Ishii, Hiroshi
Nagi, Minoru
Lim, Chang Kweng
Kato, Takanobu
Matsuoka, Saori
Aly, Hussein Hassan
Hoshino, Yasutaka
Runtuwene, Lucky Ronald
Kusagawa, Shigeru
Shimuta, Ken
Tajima, Shigeru
Takahashi, Hideyuki
Mitobe, Jiro
Nakayama, Eri
Hanaoka, Nozomu
Izumiya, Hidemasa
Shimokawa, Chikako
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Keywords COVID-19
viral infection
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Wölfel, Corman, Guggemos 2020; 581
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2024051513560144000_8.1.e000830.6
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(2024051513560144000_8.1.e000830.3) 2020; 5
References_xml – year: 2020
  ident: R10
  article-title: Predicting infectious SARS-CoV-2 from diagnostic samples
  publication-title: Clin Infect Dis
  doi: 10.1093/cid/ciaa638
– volume: 382
  start-page: 1199
  year: 2020
  ident: R1
  article-title: Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2001316
– volume: 5
  start-page: 536
  year: 2020
  ident: R3
  article-title: The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2
  publication-title: Nat Microbiol
  doi: 10.1038/s41564-020-0695-z
– volume: 39
  start-page: 1059
  year: 2020
  ident: R9
  article-title: Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards
  publication-title: Eur J Clin Microbiol Infect Dis
  doi: 10.1007/s10096-020-03913-9
– volume: 129
  start-page: 104499
  year: 2020
  ident: R7
  article-title: Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR
  publication-title: J Clin Virol
  doi: 10.1016/j.jcv.2020.104499
– volume: 73
  start-page: 304
  year: 2020
  ident: R13
  article-title: Development of genetic diagnostic methods for detection for novel coronavirus 2019(nCoV-2019) in Japan
  publication-title: Jpn J Infect Dis
  doi: 10.7883/yoken.JJID.2020.061
– volume: 117
  start-page: 7001
  year: 2020
  ident: R14
  article-title: Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.2002589117
– volume: 581
  start-page: 465
  year: 2020
  ident: R15
  article-title: Virological assessment of hospitalized patients with COVID-2019
  publication-title: Nature
  doi: 10.1038/s41586-020-2196-x
– volume: 58
  year: 2020
  ident: R12
  article-title: Comparative performance of SARS-CoV-2 detection assays using seven different primer-probe sets and one assay kit
  publication-title: J Clin Microbiol
  doi: 10.1128/JCM.00557-20
– volume: 129
  start-page: 104474
  year: 2020
  ident: R6
  article-title: Validation and verification of the Abbott RealTime SARS-CoV-2 assay analytical and clinical performance
  publication-title: J Clin Virol
  doi: 10.1016/j.jcv.2020.104474
– volume: 579
  start-page: 265
  year: 2020
  ident: R2
  article-title: A new coronavirus associated with human respiratory disease in China
  publication-title: Nature
  doi: 10.1038/s41586-020-2008-3
– volume: 25
  year: 2020
  ident: R11
  article-title: Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to may 2020
  publication-title: Euro Surveill
  doi: 10.2807/1560-7917.ES.2020.25.32.2001483
– volume: 129
  start-page: 104474
  year: 2020
  article-title: Validation and verification of the Abbott RealTime SARS-CoV-2 assay analytical and clinical performance
  publication-title: J Clin Virol
  doi: 10.1016/j.jcv.2020.104474
– volume: 117
  start-page: 7001
  year: 2020
  article-title: Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.2002589117
– volume: 39
  start-page: 1059
  year: 2020
  article-title: Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards
  publication-title: Eur J Clin Microbiol Infect Dis
  doi: 10.1007/s10096-020-03913-9
– volume: 579
  start-page: 265
  year: 2020
  article-title: A new coronavirus associated with human respiratory disease in China
  publication-title: Nature
  doi: 10.1038/s41586-020-2008-3
– volume: 5
  start-page: 536
  year: 2020
  article-title: The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2
  publication-title: Nat Microbiol
  doi: 10.1038/s41564-020-0695-z
– volume: 25
  year: 2020
  article-title: Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to may 2020
  publication-title: Euro Surveill
  doi: 10.2807/1560-7917.ES.2020.25.32.2001483
– year: 2020
  article-title: Predicting infectious SARS-CoV-2 from diagnostic samples
  publication-title: Clin Infect Dis
  doi: 10.1093/cid/ciaa638
– volume: 58
  year: 2020
  article-title: Comparative performance of SARS-CoV-2 detection assays using seven different primer-probe sets and one assay kit
  publication-title: J Clin Microbiol
  doi: 10.1128/JCM.00557-20
– volume: 73
  start-page: 304
  year: 2020
  article-title: Development of genetic diagnostic methods for detection for novel coronavirus 2019(nCoV-2019) in Japan
  publication-title: Jpn J Infect Dis
  doi: 10.7883/yoken.JJID.2020.061
– volume: 382
  start-page: 1199
  year: 2020
  article-title: Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2001316
– volume: 581
  start-page: 465
  year: 2020
  article-title: Virological assessment of hospitalized patients with COVID-2019
  publication-title: Nature
  doi: 10.1038/s41586-020-2196-x
– volume: 129
  start-page: 104499
  year: 2020
  article-title: Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR
  publication-title: J Clin Virol
  doi: 10.1016/j.jcv.2020.104499
– ident: 2024051513560144000_8.1.e000830.9
  doi: 10.1007/sl0096-020-03913-9
– volume: 58
  year: 2020
  ident: 2024051513560144000_8.1.e000830.12
  article-title: Comparative performance of SARS-CoV-2 detection assays using seven different primer-probe sets and one assay kit
  publication-title: J Clin Microbiol
  doi: 10.1128/JCM.00557-20
– volume: 129
  start-page: 104499
  year: 2020
  ident: 2024051513560144000_8.1.e000830.7
  article-title: Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR
  publication-title: J Clin Virol
  doi: 10.1016/j.jcv.2020.104499
– year: 2020
  ident: 2024051513560144000_8.1.e000830.10
  article-title: Predicting infectious SARS-CoV-2 from diagnostic samples
  publication-title: Clin Infect Dis
  doi: 10.1093/cid/ciaa638
– ident: 2024051513560144000_8.1.e000830.1
  doi: 10.1056/NEJMoa2001316
– volume: 5
  start-page: 536
  year: 2020
  ident: 2024051513560144000_8.1.e000830.3
  article-title: The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2
  publication-title: Nat Microbiol
  doi: 10.1038/s41564-020-0695-z
– ident: 2024051513560144000_8.1.e000830.5
– ident: 2024051513560144000_8.1.e000830.8
– ident: 2024051513560144000_8.1.e000830.4
– volume: 73
  start-page: 304
  year: 2020
  ident: 2024051513560144000_8.1.e000830.13
  article-title: Development of genetic diagnostic methods for detection for novel coronavirus 2019(nCoV-2019) in Japan
  publication-title: Jpn J Infect Dis
  doi: 10.7883/yoken.JJID.2020.061
– ident: 2024051513560144000_8.1.e000830.15
  doi: 10.1038/s41586-020-2196-x
– volume: 25
  year: 2020
  ident: 2024051513560144000_8.1.e000830.11
  article-title: Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to may 2020
  publication-title: Euro Surveill
  doi: 10.2807/1560-7917.ES.2020.25.32.2001483
– ident: 2024051513560144000_8.1.e000830.14
  doi: 10.1073/pnas.2002589117
– ident: 2024051513560144000_8.1.e000830.2
  doi: 10.1038/s41586-020-2008-3
– ident: 2024051513560144000_8.1.e000830.6
  doi: 10.1016/j.jcv.2020.104474
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Snippet BackgroundAn outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans...
An outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the...
Background An outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans...
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StartPage e000830
SubjectTerms Animals
Cell culture
Cell Line
Chlorocebus aethiops
Coronaviruses
COVID-19
COVID-19 - transmission
COVID-19 Nucleic Acid Testing
Cytopathogenic Effect, Viral
Disease transmission
Humans
Laboratories
Nasal Cavity - virology
Nasopharynx - virology
Public health
Respiratory Infection
Saliva - virology
SARS-CoV-2 - isolation & purification
SARS-CoV-2 - pathogenicity
Serine Endopeptidases - genetics
Severe acute respiratory syndrome coronavirus 2
Specimen Handling
Tomography
Vero Cells
viral infection
Viral infections
Viruses
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Title Assessment of SARS-CoV-2 infectivity of upper respiratory specimens from COVID-19 patients by virus isolation using VeroE6/TMPRSS2 cells
URI https://bmjopenrespres.bmj.com/content/8/1/e000830.full
https://www.ncbi.nlm.nih.gov/pubmed/33627333
https://www.proquest.com/docview/2492841101
https://www.proquest.com/docview/2493456412
https://pubmed.ncbi.nlm.nih.gov/PMC7907832
https://doaj.org/article/b9d2ad04ff3d4af5a4c6a118be36fca9
Volume 8
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